BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18414

Title: Zn(II)-AdcR   PubMed: 23138857

Deposition date: 2012-04-22 Original release date: 2013-01-03

Authors: Guerra, Alfredo; Giedroc, David

Citation: Guerra, Alfredo; Giedroc, David. "Backbone and sterospecific methyl side chain resonance assignments of the homodimeric zinc sensor AdcR (32 kDa) in the apo- and Zn(II)-bound states."  Biomol. NMR Assignments 8, 11-14 (2014).

Assembly members:
AdcR, polymer, 146 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:   Common Name: Streptococcus pneumoniae D39   Taxonomy ID: 373153   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus pneumoniae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
AdcR: MRQLAKDINAFLNEVILQAE NQHEILIGHCTSEVALTNTQ EHILMLLSEESLTNSELARR LNVSQAAVTKAIKSLVKEGM LETSKDSKDARVIFYQLTDL ARPIAEEHHHHHEHTLLTYE QVATQFTPNEQKVIQRFLTA LVGEIK

Data sets:
Data typeCount
13C chemical shifts475
15N chemical shifts139
1H chemical shifts307

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Subunit 11
2Subunit 21
3Zinc (II) ion)2

Entities:

Entity 1, Subunit 1 146 residues - Formula weight is not available

1   METARGGLNLEUALALYSASPILEASNALA
2   PHELEUASNGLUVALILELEUGLNALAGLU
3   ASNGLNHISGLUILELEUILEGLYHISCYS
4   THRSERGLUVALALALEUTHRASNTHRGLN
5   GLUHISILELEUMETLEULEUSERGLUGLU
6   SERLEUTHRASNSERGLULEUALAARGARG
7   LEUASNVALSERGLNALAALAVALTHRLYS
8   ALAILELYSSERLEUVALLYSGLUGLYMET
9   LEUGLUTHRSERLYSASPSERLYSASPALA
10   ARGVALILEPHETYRGLNLEUTHRASPLEU
11   ALAARGPROILEALAGLUGLUHISHISHIS
12   HISHISGLUHISTHRLEULEUTHRTYRGLU
13   GLNVALALATHRGLNPHETHRPROASNGLU
14   GLNLYSVALILEGLNARGPHELEUTHRALA
15   LEUVALGLYGLUILELYS

Entity 2, Zinc (II) ion) - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: AdcR, [U-100% 13C; U-100% 15N; U-80% 2H], 600 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.00; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)CBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis, peak picking

NMR spectrometers:

  • Varian VNMRS 600 MHz
  • Varian VNMRS 800 MHz

Related Database Links:

BMRB 18413
PDB
EMBL CAA96184 CAR69937 CBJ23263 CBW33569 CBW35616
GB AAK76226 AAL00780 ABJ54931 ACA36761 ACB91392
REF NP_359569 WP_000042198 WP_001249307 WP_001249308 WP_001249309
SP Q04I02

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts