BMRB Entry 18562
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18562
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Title: Backbone and side-chain assignments of an effector membrane localization domain from Vibrio vulnificus MARTX toxin PubMed: 23765285
Deposition date: 2012-06-30 Original release date: 2014-02-11
Authors: Brothers, Michael; Geissler, Brett; Hsiao, Grant; Wilson, Brenda; Satchell, Karla; Rienstra, Chad
Citation: Brothers, Michael; Geissler, Brett; Hisao, Grant; Wilson, Brenda; Satchell, Karla; Rienstra, Chad. "Backbone and side-chain assignments of an effector membrane localization domain from Vibrio vulnificus MARTX toxin." Biomol. NMR Assignments ., .-. (2013).
Assembly members:
VvMARTX_MLD, polymer, 90 residues, Formula weight is not available
Natural source: Common Name: g-proteobacteria Taxonomy ID: 672 Superkingdom: Bacteria Kingdom: not available Genus/species: Vibrio Vulnificus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
VvMARTX_MLD: MGQIFTVQELKERAKVFAKP
IGASYQGILDQLDLVHQAKG
RDQIAASFELNKKINDYIAE
HPTSGRNQALTQLKEQVTSA
LGLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 323 |
| 15N chemical shifts | 73 |
| 1H chemical shifts | 427 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VvMARTX MLD | 1 |
Entities:
Entity 1, VvMARTX MLD 90 residues - Formula weight is not available
| 1 | MET | GLY | GLN | ILE | PHE | THR | VAL | GLN | GLU | LEU | |
| 2 | LYS | GLU | ARG | ALA | LYS | VAL | PHE | ALA | LYS | PRO | |
| 3 | ILE | GLY | ALA | SER | TYR | GLN | GLY | ILE | LEU | ASP | |
| 4 | GLN | LEU | ASP | LEU | VAL | HIS | GLN | ALA | LYS | GLY | |
| 5 | ARG | ASP | GLN | ILE | ALA | ALA | SER | PHE | GLU | LEU | |
| 6 | ASN | LYS | LYS | ILE | ASN | ASP | TYR | ILE | ALA | GLU | |
| 7 | HIS | PRO | THR | SER | GLY | ARG | ASN | GLN | ALA | LEU | |
| 8 | THR | GLN | LEU | LYS | GLU | GLN | VAL | THR | SER | ALA | |
| 9 | LEU | GLY | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: DSS .01 mM; EDTA 2 mM; NaCl 500 mM; VvMARTX MLD, [U-99% 13C; U-99% 15N], 1 mM; Tris-HCl 50 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.5 M; pH: 7.4; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ v2.2, Varian - collection
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts