BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18657

Title: 1H,13C,15N assignments for an isoform of the type III antifreeze protein from notched-fin eelpout   PubMed: 23288342

Deposition date: 2012-08-12 Original release date: 2013-02-13

Authors: Kumeta, Hiroyuki; Ogura, Kenji; Nishimiya, Yoshiyuki; Miura, Ai; Inagaki, Fuyuhiko; Tsuda, Sakae

Citation: Kumeta, Hiroyuki; Ogura, Kenji; Nishimiya, Yoshiyuki; Miura, Ai; Inagaki, Fuyuhiko; Tsuda, Sakae. "NMR structure note: a defective isoform and its activity-improved variant of a type III antifreeze protein from Zoarces elongates Kner."  J. Biomol. NMR 55, 225-230 (2013).

Assembly members:
nfeAFP11, polymer, 67 residues, 7071.434 Da.

Natural source:   Common Name: not available   Taxonomy ID: 291231   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Zoarces elongatus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
nfeAFP11: MNQESVVAAQLIPINTALTL VMMTTRVVSPTGIPAEDIPR LISMQVNQVVPMGTTLMPDM VKGYAPA

Data sets:
Data typeCount
13C chemical shifts292
15N chemical shifts67
1H chemical shifts511

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1nfeAFP111

Entities:

Entity 1, nfeAFP11 67 residues - 7071.434 Da.

1   METASNGLNGLUSERVALVALALAALAGLN
2   LEUILEPROILEASNTHRALALEUTHRLEU
3   VALMETMETTHRTHRARGVALVALSERPRO
4   THRGLYILEPROALAGLUASPILEPROARG
5   LEUILESERMETGLNVALASNGLNVALVAL
6   PROMETGLYTHRTHRLEUMETPROASPMET
7   VALLYSGLYTYRALAPROALA

Samples:

sample_1: nfeAFP11, [U-100% 13C; U-100% 15N], 2 mM; potassium chloride 20 mM; DSS 0.1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

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