BMRB Entry 18691
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18691
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Title: Backbone 1H, 13C, and 15N backbone resonance assignments of the TPR1 domain of mouse STI1 PubMed: 23070844
Deposition date: 2012-08-30 Original release date: 2012-10-18
Authors: Maciejewski, Andrzej; Choy, Wing-Yiu
Citation: Maciejewski, Andrzej; Prado, Marco; Choy, Wing-Yiu. "1H, 15N, and 13C backbone resonance assignments of the TPR1 and TPR2A domains of mouse STI1." Biomol. NMR Assignments 7, 305-310 (2013).
Assembly members:
tpr1, polymer, 118 residues, Formula weight is not available
Natural source: Common Name: House mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
tpr1: GEQVNELKEKGNKALSAGNI
DDALQCYSEAIKLDPQNHVL
YSNRSAAYAKKGDYQKAYED
GCKTVDLKPDWGKGYSRKAA
ALEFLNRFEEAKRTYEEGLK
HEANNLQLKEGLQNMEAR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 198 |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 111 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | tpr1 | 1 |
Entities:
Entity 1, tpr1 118 residues - Formula weight is not available
| 1 | GLY | GLU | GLN | VAL | ASN | GLU | LEU | LYS | GLU | LYS | ||||
| 2 | GLY | ASN | LYS | ALA | LEU | SER | ALA | GLY | ASN | ILE | ||||
| 3 | ASP | ASP | ALA | LEU | GLN | CYS | TYR | SER | GLU | ALA | ||||
| 4 | ILE | LYS | LEU | ASP | PRO | GLN | ASN | HIS | VAL | LEU | ||||
| 5 | TYR | SER | ASN | ARG | SER | ALA | ALA | TYR | ALA | LYS | ||||
| 6 | LYS | GLY | ASP | TYR | GLN | LYS | ALA | TYR | GLU | ASP | ||||
| 7 | GLY | CYS | LYS | THR | VAL | ASP | LEU | LYS | PRO | ASP | ||||
| 8 | TRP | GLY | LYS | GLY | TYR | SER | ARG | LYS | ALA | ALA | ||||
| 9 | ALA | LEU | GLU | PHE | LEU | ASN | ARG | PHE | GLU | GLU | ||||
| 10 | ALA | LYS | ARG | THR | TYR | GLU | GLU | GLY | LEU | LYS | ||||
| 11 | HIS | GLU | ALA | ASN | ASN | LEU | GLN | LEU | LYS | GLU | ||||
| 12 | GLY | LEU | GLN | ASN | MET | GLU | ALA | ARG |
Samples:
sample_1: sodium phosphate 50 mM; sodium chloride 50 mM; DTT 1 mM; DSS 100 uM; tpr1, [U-13C; U-15N], 0.5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts