BMRB Entry 18748
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18748
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Title: Backbone chemical shift assignments of the C-terminal SH3 domain of Grb2 PubMed: 22726438
Deposition date: 2012-10-01 Original release date: 2012-11-02
Authors: Ghosh, Ragini; Ladbury, John
Citation: Ghosh, Ragini; Ladbury, John. "Inhibition of Basal FGF Receptor Signaling by Dimeric Grb2" Cell 149, 1514-1524 (2012).
Assembly members:
Grb2_C-terminal_SH3_domain, polymer, 74 residues, 8599 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Grb2_C-terminal_SH3_domain: MRGSHHHHHHGSQQPTYVQA
LFDFDPQEDGELGFRRGDFI
HVMDNSDPNWWKGACHGQTG
MFPRNYVTPVNRNV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 158 |
| 15N chemical shifts | 50 |
| 1H chemical shifts | 50 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Grb2 C-terminal SH3 domain | 1 |
Entities:
Entity 1, Grb2 C-terminal SH3 domain 74 residues - 8599 Da.
| 1 | MET | ARG | GLY | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | GLY | SER | GLN | GLN | PRO | THR | TYR | VAL | GLN | ALA | ||||
| 3 | LEU | PHE | ASP | PHE | ASP | PRO | GLN | GLU | ASP | GLY | ||||
| 4 | GLU | LEU | GLY | PHE | ARG | ARG | GLY | ASP | PHE | ILE | ||||
| 5 | HIS | VAL | MET | ASP | ASN | SER | ASP | PRO | ASN | TRP | ||||
| 6 | TRP | LYS | GLY | ALA | CYS | HIS | GLY | GLN | THR | GLY | ||||
| 7 | MET | PHE | PRO | ARG | ASN | TYR | VAL | THR | PRO | VAL | ||||
| 8 | ASN | ARG | ASN | VAL |
Samples:
sample_1: Grb2 C-terminal SH3 domain, [U-100% 13C; U-100% 15N], 0.3 mM; sodium phosphate 25 mM; sodium chloride 140 mM; DTT 2 mM; H2O 90%; D2O 10%
sample_2: Grb2 C-terminal SH3 domain, [U-100% 15N], 0.3 mM; sodium phosphate 25 mM; sodium chloride 140 mM; DTT 2 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.215 M; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
ANALYSIS, CCPN - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 500 MHz
Related Database Links:
| PDB | |
| EMBL | CAA44664 |
| GB | ELK30972 ELW68317 ERE69131 |
| REF | XP_006728173 XP_006940642 XP_008766679 XP_010340838 XP_010629093 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts