BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18782

Title: Solution structure of the QUA1 dimerization domain of pXqua, the Xenopus ortholog of Quaking.   PubMed: 23520467

Deposition date: 2012-10-11 Original release date: 2015-07-03

Authors: Ali, M.; Broadhurst, Richard

Citation: Ali, M.; Broadhurst, Richard. "Solution structure of the QUA1 dimerization domain of pXqua, the Xenopus ortholog of Quaking"  PLOS ONE 8, e57345-e57345 (2013).

Assembly members:
PROTEIN_QUAKING-A, polymer, 52 residues, 6110.9728 Da.

Natural source:   Common Name: AFRICAN CLAWED FROG   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PROTEIN_QUAKING-A: GSKEKPKPTPDYLMQLMNDK KLMSSLPNFSGIFTHLERLL DEEISRVRKDMY

Data sets:
Data typeCount
13C chemical shifts246
15N chemical shifts52
1H chemical shifts392

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PROTEIN QUAKING-A1

Entities:

Entity 1, PROTEIN QUAKING-A 52 residues - 6110.9728 Da.

1   GLYSERLYSGLULYSPROLYSPROTHRPRO
2   ASPTYRLEUMETGLNLEUMETASNASPLYS
3   LYSLEUMETSERSERLEUPROASNPHESER
4   GLYILEPHETHRHISLEUGLUARGLEULEU
5   ASPGLUGLUILESERARGVALARGLYSASP
6   METTYR

Samples:

sample_1: PROTEIN QUAKING-A, [U-13C; U-15N], 1.0 mM

sample_conditions_1: ionic strength: 150.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
[1H-15N]-HSQCsample_1solutionsample_conditions_1
15N-TOCSY-HSQCsample_1solutionsample_conditions_1
15N-NOESY-HSQCsample_1solutionsample_conditions_1
13C-NOESY-HSQCsample_1solutionsample_conditions_1
HNCAsample_1solutionsample_conditions_1
HN(CO)CAsample_1solutionsample_conditions_1
HNCACBsample_1solutionsample_conditions_1
CBCA(CO)NHsample_1solutionsample_conditions_1
HNCOsample_1solutionsample_conditions_1
HBHA(CO)NHsample_1solutionsample_conditions_1
HCCH-TOCSYsample_1solutionsample_conditions_1

Software:

ARIA-CNS vany, Nilges et al - chemical shift assignment

AutoDep v4.3, PDBe - chemical shift assignment

CCPN_Analysis vany, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

UNP QKIA_XENLA
PDB

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts