BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18847

Title: Structure of stacked G-quadruplex formed by human TERRA sequence in potassium solution   PubMed: 23445442

Deposition date: 2012-11-20 Original release date: 2013-03-01

Authors: Martadinata, Herry; Phan, Anh Tuan

Citation: Martadinata, Herry; Phan, Anh Tuan. "Structure of Human Telomeric RNA (TERRA): Stacking of Two G-Quadruplex Blocks in K(+) Solution."  Biochemistry 52, 2176-2183 (2013).

Assembly members:
RNA_(5'-R(*GP*GP*GP*UP*UP*AP*GP*GP*GP*U)-3'), polymer, 10 residues, 3274.003 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
RNA_(5'-R(*GP*GP*GP*UP*UP*AP*GP*GP*GP*U)-3'): GGGUUAGGGU

Data sets:
Data typeCount
1H chemical shifts232

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GGGUUAGGGU, chain 11
2GGGUUAGGGU, chain 21
3GGGUUAGGGU, chain 31
4GGGUUAGGGU, chain 41

Entities:

Entity 1, GGGUUAGGGU, chain 1 10 residues - 3274.003 Da.

1   GGGUUAGGGU

Samples:

10-nt_TERRA: RNA (5, -R(*GP*GP*GP*UP*UP*AP*GP*GP*GP*U)-3')', ; 2, K+ solution, ;

sample_conditions_1: ionic strength: 90 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC aliphatic10-nt_TERRAisotropicsample_conditions_1
2D 1H-13C HSQC aromatic10-nt_TERRAisotropicsample_conditions_1
2D 1H-1H TOCSY10-nt_TERRAisotropicsample_conditions_1
2D 1H-1H COSY10-nt_TERRAisotropicsample_conditions_1
2D 1H-1H NOESY10-nt_TERRAisotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

FELIX, Accelrys Software Inc. - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz