BMRB Entry 18890
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18890
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Title: CD3g cytosolic domain PubMed: 23667548
Deposition date: 2012-12-11 Original release date: 2013-10-15
Authors: Mayzel, Maxim
Citation: Isaksson, Linnea; Mayzel, Maxim; Saline, Maria; Pedersen, Anders; Brutscher, Bernhard; Karlsson, Goran; Orekhov, Vladislav. "Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains" PLoS One 8, e62947-e62947 (2013).
Assembly members:
CD3g, polymer, 46 residues, 5426.8739 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Wheat germ - cell freeE. coli - cell free
Entity Sequences (FASTA):
CD3g: SLQDGVRQSRASDKQTLLPN
DQLYQPLKDREDDQYSHLQG
NQLRRN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 136 |
| 15N chemical shifts | 43 |
| 1H chemical shifts | 43 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CD3g | 1 |
Entities:
Entity 1, CD3g 46 residues - 5426.8739 Da.
| 1 | SER | LEU | GLN | ASP | GLY | VAL | ARG | GLN | SER | ARG | ||||
| 2 | ALA | SER | ASP | LYS | GLN | THR | LEU | LEU | PRO | ASN | ||||
| 3 | ASP | GLN | LEU | TYR | GLN | PRO | LEU | LYS | ASP | ARG | ||||
| 4 | GLU | ASP | ASP | GLN | TYR | SER | HIS | LEU | GLN | GLY | ||||
| 5 | ASN | GLN | LEU | ARG | ARG | ASN |
Samples:
Native: CD3g, [U-100% 13C; U-100% 15N], 0.10 mM; DTT 2.00 mM; EDTA 1.00 mM; phosphate buffer 20.00 mM; azide 0.02%; Complete EDTA-Free, [U-100% 15N], 1.00 x
1: ionic strength: 0.020 M; pH: 6.800; pressure: 1.000 atm; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HN(CA)CO | Native | isotropic | 1 |
| 3D HNCA | Native | isotropic | 1 |
| 3D HNCACB | Native | isotropic | 1 |
| 3D HNCO | Native | isotropic | 1 |
| 3D HN(CO)CA | Native | isotropic | 1 |
| 3D HN(CO)CACB | Native | isotropic | 1 |
| 2D 1H-15N HSQC/HMQC | Native | isotropic | 1 |
Software:
AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - Automatic assignment
ANALYSIS v2.2, CCPN - refinement
MDDNMR v2.1, Swedish NMR Center, University of Gothenburg - Spectrum processing
TANSY v1.0, Swedish NMR Center, University of Gothenburg - Spectra processing & analysis
NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing
NMR spectrometers:
- Varian UnityInova 600 MHz
Related Database Links:
| UNP | P09693 |
| DBJ | BAB71848 BAD97034 BAF98786 BAG36681 BAJ16153 |
| EMBL | CAA27764 CAA29428 |
| GB | AAI13831 ACA05963 AIC48442 EAW67368 EHH23478 |
| REF | NP_000064 NP_001253854 NP_001270839 XP_002754529 XP_003253274 |
| SP | P09693 Q95LI7 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts