BMRB Entry 18918
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18918
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Title: Chemical shifts assignment for the unfolded state of the N-terminal domain of ribosomal protein L9 (NTL9) V3AI4A double mutant in 8.3 M urea PubMed: 23480024
Deposition date: 2012-12-22 Original release date: 2013-05-30
Authors: Meng, Wenli; Raleigh, Daniel
Citation: Meng, Wenli; Luan, Bowu; Lyle, Nicholas; Pappu, Rohit; Raleigh, Daniel. "The denatured state ensemble contains significant local and long-range structure under native conditions: analysis of the N-terminal domain of ribosomal protein L9." Biochemistry 52, 2662-2671 (2013).
Assembly members:
NTL9_V3AI4A_double_mutant, polymer, 56 residues, Formula weight is not available
Natural source: Common Name: Geobacillus stearothermophilus Taxonomy ID: 1422 Superkingdom: Bacteria Kingdom: not available Genus/species: Geobacillus stearothermophilus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
NTL9_V3AI4A_double_mutant: MKAAFLKDVKGKGKKGEIKN
VADGYANNFLFKQGLAIEAT
PANLKALEAQKQKEQR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 160 |
| 15N chemical shifts | 54 |
| 1H chemical shifts | 54 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NTL9_V3AI4A | 1 |
Entities:
Entity 1, NTL9_V3AI4A 56 residues - Formula weight is not available
| 1 | MET | LYS | ALA | ALA | PHE | LEU | LYS | ASP | VAL | LYS | ||||
| 2 | GLY | LYS | GLY | LYS | LYS | GLY | GLU | ILE | LYS | ASN | ||||
| 3 | VAL | ALA | ASP | GLY | TYR | ALA | ASN | ASN | PHE | LEU | ||||
| 4 | PHE | LYS | GLN | GLY | LEU | ALA | ILE | GLU | ALA | THR | ||||
| 5 | PRO | ALA | ASN | LEU | LYS | ALA | LEU | GLU | ALA | GLN | ||||
| 6 | LYS | GLN | LYS | GLU | GLN | ARG |
Samples:
sample_1: NTL9 V3AI4A double mutant, [U-99% 13C; U-99% 15N], 0.5 mM; urea 8.3 M
sample_conditions_1: ionic strength: 0.12 M; pH: 5.5; pressure: 1 atm; temperature: 285 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts