BMRB Entry 18940
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18940
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Title: Structure of the S2 neuropeptide from the starfish Asterias rubens. PubMed: 24200677
Deposition date: 2013-01-06 Original release date: 2014-04-22
Authors: Jones, Christopher
Citation: Otara, Claire; Jones, Christopher; Younan, Nadine; Viles, John; Elphick, Maurice. "Structural analysis of the starfish SALMFamide neuropeptides S1 and S2: the N-terminal region of S2 facilitates self-association." Biochim. Biophys. Acta 1844, 358-365 (2014).
Assembly members:
S2, polymer, 12 residues, 1276.365 Da.
Natural source: Common Name: European starfish Taxonomy ID: 7604 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Asterias rubens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
S2: SGPYSFNSGLTF
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 73 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | S2 | 1 |
Entities:
Entity 1, S2 12 residues - 1276.365 Da.
| 1 | SER | GLY | PRO | TYR | SER | PHE | ASN | SER | GLY | LEU | ||||
| 2 | THR | PHE |
Samples:
sample_1: S2 2 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 5.6; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
XEASY, Bartels et al. - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz