BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18948

Title: STRUCTURE OF LASSO PEPTIDE ASTEXIN-1(19)   PubMed: 23601645

Deposition date: 2013-01-14 Original release date: 2014-02-14

Authors: Zimmermann, M.; Hegemann, J.; Xie, X.; Marahiel, M.

Citation: Zimmermann, Marcel; Hegemann, Julian; Xie, Xiulan; Marahiel, Mohamed. "The astexin-1 lasso peptides: biosynthesis, stability, and structural studies."  Chem. Biol. 20, 558-569 (2013).

Assembly members:
ASTEXIN-1(19), polymer, 19 residues, 2113.240 Da.

Natural source:   Common Name: A-PROTEOBACTERIA   Taxonomy ID: 78587   Superkingdom: BACTERIA   Kingdom: not available   Genus/species: ASTICCACAULIS EXCENTRICUS

Experimental source:   Production method: recombinant technology   Host organism: ASTICCACAULIS EXCENTRICUS

Entity Sequences (FASTA):
ASTEXIN-1(19): GLSQGVEPDIGQTYFEESR

Data sets:
Data typeCount
1H chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ASTEXIN-1(19)1

Entities:

Entity 1, ASTEXIN-1(19) 19 residues - 2113.240 Da.

1   GLYLEUSERGLNGLYVALGLUPROASPILE
2   GLYGLNTHRTYRPHEGLUGLUSERARG

Samples:

sample_1: ASTEXIN-1(19) 5.7 mM; H2O 90%; D2O 10%

sample_conditions_1: pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 18481 25754
PDB
GB ADU13884