BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19032

Title: Analysis of the structural and molecular basis of voltage-sensitive sodium channel inhibition by the spider toxin, Huwentoxin-IV ( -TRTX-Hh2a).   PubMed: 23760503

Deposition date: 2013-02-12 Original release date: 2013-06-17

Authors: Gibbs, Al; Minassian, Natali; Flinspach, Mack; Wickenden, Alan

Citation: Minassian, Natali; Gibbs, Alan; Shih, Amy; Liu, Yi; Neff, Robert; Sutton, Steven; Mirzadegan, Tara; Connor, Judith; Fellows, Ross; Husovsky, Matthew; Nelson, Serena; Hunter, Michael; Flinspach, Mack; Wickenden, Alan. "Analysis of the Structural and Molecular Basis of Voltage-sensitive Sodium Channel Inhibition by the Spider Toxin Huwentoxin-IV (-TRTX-Hh2a)."  J. Biol. Chem. 288, 22707-22720 (2013).

Assembly members:
Huwentoxin-IV, polymer, 35 residues, 4062.773 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology   Host organism: Homo sapiens

Entity Sequences (FASTA):
Huwentoxin-IV: ECLEIFKACNPSNDQCCKSS KLVCSRKTRWCAYQI

Data sets:
Data typeCount
1H chemical shifts187

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Huwentoxin-IV1

Entities:

Entity 1, Huwentoxin-IV 35 residues - 4062.773 Da.

1   GLUCYSLEUGLUILEPHELYSALACYSASN
2   PROSERASNASPGLNCYSCYSLYSSERSER
3   LYSLEUVALCYSSERARGLYSTHRARGTRP
4   CYSALATYRGLNILE

Samples:

sample_1: Huwentoxin-IV 2 mM; sodium phosphate 20 mM; sodium azide 150 uM; EDTA, [U-99% 2H], 100 uM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 950 MHz

Related Database Links:

BMRB 19026
PDB
GB AAP33074 ABY77744 ABY77746
SP P83303