BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19051

Title: 1H, 13C, 15N assignment of Voltage-gated calcium channel beta 4 subunit core domains   PubMed: 23712306

Deposition date: 2013-02-22 Original release date: 2013-09-11

Authors: Xu, Xingfu; Horne, William

Citation: Xu, Xingfu; Horne, William. "(1)H, (13)C, and (15)N backbone resonance assignments of the 37kDa voltage-gated Ca(2+) channel 4 subunit core SH3-GK domains."  Biomol. NMR Assignments ., .-. (2013).

Assembly members:
calcium_channel_beta_subunit, polymer, 352 residues, Formula weight is not available

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
calcium_channel_beta_subunit: MGSSHHHHHHSSGLVPRGHS MGSADSYTSRPSDSDVSLEE DREAIRQEREQQAAIQLERA KSKPVAFAVKTNVSYSGALD EDVPVPSTAISFDAKDFLHI KEKYNNDWWIGRLVKEGSEI GFIPSPLRLENIRIQQEQKR GSSAKQKQKVTEHIPPYDVV PSMRPVVLVGPSLKGYEVTD MMQKALFDFLKHRFDGRISI TRVTADISLAKRSVLNNPSK RAIIERSNTRSSLAEVQSEI ERIFELARSLQLVVLDADTI NHPAQLIKTSLAPIIVHVKV SSPKVLQRLIKSRGKSQSKH LNVQLVAADKLAQCPPEMFD VILDENQLEDACEHLGEYLE AYWRATHTTSST

Data sets:
Data typeCount
13C chemical shifts900
15N chemical shifts292
1H chemical shifts290

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calcium_channel_beta_subunit1

Entities:

Entity 1, calcium_channel_beta_subunit 352 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYHISSER
3   METGLYSERALAASPSERTYRTHRSERARG
4   PROSERASPSERASPVALSERLEUGLUGLU
5   ASPARGGLUALAILEARGGLNGLUARGGLU
6   GLNGLNALAALAILEGLNLEUGLUARGALA
7   LYSSERLYSPROVALALAPHEALAVALLYS
8   THRASNVALSERTYRSERGLYALALEUASP
9   GLUASPVALPROVALPROSERTHRALAILE
10   SERPHEASPALALYSASPPHELEUHISILE
11   LYSGLULYSTYRASNASNASPTRPTRPILE
12   GLYARGLEUVALLYSGLUGLYSERGLUILE
13   GLYPHEILEPROSERPROLEUARGLEUGLU
14   ASNILEARGILEGLNGLNGLUGLNLYSARG
15   GLYSERSERALALYSGLNLYSGLNLYSVAL
16   THRGLUHISILEPROPROTYRASPVALVAL
17   PROSERMETARGPROVALVALLEUVALGLY
18   PROSERLEULYSGLYTYRGLUVALTHRASP
19   METMETGLNLYSALALEUPHEASPPHELEU
20   LYSHISARGPHEASPGLYARGILESERILE
21   THRARGVALTHRALAASPILESERLEUALA
22   LYSARGSERVALLEUASNASNPROSERLYS
23   ARGALAILEILEGLUARGSERASNTHRARG
24   SERSERLEUALAGLUVALGLNSERGLUILE
25   GLUARGILEPHEGLULEUALAARGSERLEU
26   GLNLEUVALVALLEUASPALAASPTHRILE
27   ASNHISPROALAGLNLEUILELYSTHRSER
28   LEUALAPROILEILEVALHISVALLYSVAL
29   SERSERPROLYSVALLEUGLNARGLEUILE
30   LYSSERARGGLYLYSSERGLNSERLYSHIS
31   LEUASNVALGLNLEUVALALAALAASPLYS
32   LEUALAGLNCYSPROPROGLUMETPHEASP
33   VALILELEUASPGLUASNGLNLEUGLUASP
34   ALACYSGLUHISLEUGLYGLUTYRLEUGLU
35   ALATYRTRPARGALATHRHISTHRTHRSER
36   SERTHR

Samples:

sample_1: calcium channel beta subunit, [U-13C; U-15N; U-2H], 0.5 – 0.6 mM; D2O 10%; sodium acetate 0.02%; potassium phosphate 50 mM; sodium chloride 100 mM; TCEP 2 mM; H2O 90%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

GB KFQ57190
REF NP_001272357 XP_004316422 XP_006497705 XP_007629197 XP_007652372

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts