BMRB Entry 19055
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19055
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Title: ns5a D2 con1
Deposition date: 2013-02-26 Original release date: 2017-01-30
Authors: Badillo, Aurelie; Penin, Francois
Citation: Badillo, Aurelie; Hanoulle, Xavier; Penin, Francois. "ns5a D2 con1" Biochemistry ., .-..
Assembly members:
ns5a_d2_con1, polymer, 96 residues, Formula weight is not available
Natural source: Common Name: Hepatitis C virus Taxonomy ID: 11103 Superkingdom: Viruses Kingdom: not available Genus/species: Hepacivirus not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ns5a_d2_con1: DSPDADLIEANLLWRQEMGG
NITRVESENKVVILDSFEPL
QAEEDEREVSVPAEILRRSR
KFPRAMPIWARPDYNPPLLE
SWKDPDYVPPVVHGLQ
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 285 |
15N chemical shifts | 85 |
1H chemical shifts | 85 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | ns5a d2 con1 | 1 |
Entities:
Entity 1, ns5a d2 con1 96 residues - Formula weight is not available
1 | ASP | SER | PRO | ASP | ALA | ASP | LEU | ILE | GLU | ALA | ||||
2 | ASN | LEU | LEU | TRP | ARG | GLN | GLU | MET | GLY | GLY | ||||
3 | ASN | ILE | THR | ARG | VAL | GLU | SER | GLU | ASN | LYS | ||||
4 | VAL | VAL | ILE | LEU | ASP | SER | PHE | GLU | PRO | LEU | ||||
5 | GLN | ALA | GLU | GLU | ASP | GLU | ARG | GLU | VAL | SER | ||||
6 | VAL | PRO | ALA | GLU | ILE | LEU | ARG | ARG | SER | ARG | ||||
7 | LYS | PHE | PRO | ARG | ALA | MET | PRO | ILE | TRP | ALA | ||||
8 | ARG | PRO | ASP | TYR | ASN | PRO | PRO | LEU | LEU | GLU | ||||
9 | SER | TRP | LYS | ASP | PRO | ASP | TYR | VAL | PRO | PRO | ||||
10 | VAL | VAL | HIS | GLY | LEU | GLN |
Samples:
sample_1: ns5a d2 con1, [U-100% 13C; U-100% 15N], 0.5 ± 0.1 mM; H2O 95 ± 0.1 %; D2O 5 ± 0.1 %
sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts