BMRB Entry 19125
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19125
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Title: Molecular Basis of Histone Acetyllysine Recognition by the BRPF1 Bromodomain. PubMed: 24333487
Deposition date: 2013-03-28 Original release date: 2014-02-12
Authors: Poplawski, Amanda; Hu, Kaifeng; Lee, Woonghee; Peng, Danni; Carlson, Samuel; Neuhardt, Elizabeth; Shi, Xiaobing; Markley, John; Glass, Karen
Citation: Poplawski, Amanda; Hu, Kaifeng; Lee, Woonghee; Natesan, Senthil; Peng, Danni; Carlson, Samuel; Shi, Xiaobing; Balaz, Stefan; Markley, John; Glass, Karen. "Molecular Insights into the Recognition of N-Terminal Histone Modifications by the BRPF1 Bromodomain." J. Mol. Biol. 426, 1661-1676 (2014).
Assembly members:
BRPF1_bromodomain, polymer, 117 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
BRPF1_bromodomain: GPLQLTPFLILLRKTLEQLQ
EKDTGNIFSEPVPLSEVPDY
LDHIKKPMDFFTMKQNLEAY
RYLNFDDFEEDFNLIVSNCL
KYNAKDTIFYRAAVRLREQG
GAVLRQARRQAEKMGID
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 317 |
| 15N chemical shifts | 99 |
| 1H chemical shifts | 99 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BRPF1 | 1 |
Entities:
Entity 1, BRPF1 117 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLN | LEU | THR | PRO | PHE | LEU | ILE | ||||
| 2 | LEU | LEU | ARG | LYS | THR | LEU | GLU | GLN | LEU | GLN | ||||
| 3 | GLU | LYS | ASP | THR | GLY | ASN | ILE | PHE | SER | GLU | ||||
| 4 | PRO | VAL | PRO | LEU | SER | GLU | VAL | PRO | ASP | TYR | ||||
| 5 | LEU | ASP | HIS | ILE | LYS | LYS | PRO | MET | ASP | PHE | ||||
| 6 | PHE | THR | MET | LYS | GLN | ASN | LEU | GLU | ALA | TYR | ||||
| 7 | ARG | TYR | LEU | ASN | PHE | ASP | ASP | PHE | GLU | GLU | ||||
| 8 | ASP | PHE | ASN | LEU | ILE | VAL | SER | ASN | CYS | LEU | ||||
| 9 | LYS | TYR | ASN | ALA | LYS | ASP | THR | ILE | PHE | TYR | ||||
| 10 | ARG | ALA | ALA | VAL | ARG | LEU | ARG | GLU | GLN | GLY | ||||
| 11 | GLY | ALA | VAL | LEU | ARG | GLN | ALA | ARG | ARG | GLN | ||||
| 12 | ALA | GLU | LYS | MET | GLY | ILE | ASP |
Samples:
sample_1: Tris HCl 20 mM; NaCl 150 mM; DTT 10 mM; BRPF1_bromodomain, [U-13C; U-15N], 0 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
ADAPT-NMR, (ADAPT-NMR)-NMRFAM - chemical shift assignment, collection, peak picking
NMR spectrometers:
- Bruker Avance III 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAC31528 BAE33691 BAG10583 BAG57257 |
| EMBL | CAD28495 |
| GB | AAB02119 AAF19605 AAH05647 AAH46521 AAI38362 |
| REF | NP_001178501 NP_001269055 NP_001269056 NP_004625 NP_084454 |
| SP | P55201 |
| TPG | DAA17219 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts