BMRB Entry 19134
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19134
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Title: Backbone assignments of 8M urea-denatured YbeA from Escherichia coli PubMed: 23821130
Deposition date: 2013-04-02 Original release date: 2014-09-26
Authors: Hsieh, Shu-Ju Micky; Hsu, Shang-Te Danny
Citation: Hsieh, Shu-Ju Micky; Mallam, Anna; Jackson, Sophie; Hsu, Shang-Te Danny. "Backbone NMR assignments of a topologically knotted protein in urea-denatured state." Biomol. NMR Assign. 8, 283-285 (2014).
Assembly members:
YbeA, polymer, 155 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
YbeA: MKLQLVAVGTKMPDWVQTGF
TEYLRRFPKDMPFELIEIPA
GKRGKNADIKRILDKEGEQM
LAAAGKNRIVTLDIPGKPWD
TPQLAAELERWKLDGRDVSL
LIGGPEGLSPACKAAAEQSW
SLSALTLPHPLVRVLVAESL
YRAWSITTNHPYHRE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 422 |
| 15N chemical shifts | 135 |
| 1H chemical shifts | 135 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | YbeA | 1 |
Entities:
Entity 1, YbeA 155 residues - Formula weight is not available
| 1 | MET | LYS | LEU | GLN | LEU | VAL | ALA | VAL | GLY | THR | ||||
| 2 | LYS | MET | PRO | ASP | TRP | VAL | GLN | THR | GLY | PHE | ||||
| 3 | THR | GLU | TYR | LEU | ARG | ARG | PHE | PRO | LYS | ASP | ||||
| 4 | MET | PRO | PHE | GLU | LEU | ILE | GLU | ILE | PRO | ALA | ||||
| 5 | GLY | LYS | ARG | GLY | LYS | ASN | ALA | ASP | ILE | LYS | ||||
| 6 | ARG | ILE | LEU | ASP | LYS | GLU | GLY | GLU | GLN | MET | ||||
| 7 | LEU | ALA | ALA | ALA | GLY | LYS | ASN | ARG | ILE | VAL | ||||
| 8 | THR | LEU | ASP | ILE | PRO | GLY | LYS | PRO | TRP | ASP | ||||
| 9 | THR | PRO | GLN | LEU | ALA | ALA | GLU | LEU | GLU | ARG | ||||
| 10 | TRP | LYS | LEU | ASP | GLY | ARG | ASP | VAL | SER | LEU | ||||
| 11 | LEU | ILE | GLY | GLY | PRO | GLU | GLY | LEU | SER | PRO | ||||
| 12 | ALA | CYS | LYS | ALA | ALA | ALA | GLU | GLN | SER | TRP | ||||
| 13 | SER | LEU | SER | ALA | LEU | THR | LEU | PRO | HIS | PRO | ||||
| 14 | LEU | VAL | ARG | VAL | LEU | VAL | ALA | GLU | SER | LEU | ||||
| 15 | TYR | ARG | ALA | TRP | SER | ILE | THR | THR | ASN | HIS | ||||
| 16 | PRO | TYR | HIS | ARG | GLU |
Samples:
sample_1: YbeA, [U-13C; U-15N], 350 uM; D2O 10%; H2O 90%; TRIS 50 mM; potassium chloride 200 mM; glycerol 10%; urea 7.2 M
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAA35283 BAB34097 BAG76228 BAI24039 BAI29506 |
| EMBL | CAA28200 CAD05118 CAP75135 CAQ31111 CAQ89966 |
| GB | AAB40836 AAC73737 AAG54970 AAL19592 AAN42281 |
| PIR | AD0581 |
| REF | NP_308701 NP_415169 NP_455217 NP_459633 NP_706574 |
| SP | A1A8R1 A4W822 A7ZJ25 A7ZXR2 A8AJG7 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts