BMRB Entry 19140
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19140
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Title: Dynorphin A R6W PubMed: 23705820
Deposition date: 2013-04-04 Original release date: 2013-05-06
Authors: Bjorneras, Johannes; Graslund, Astrid; Maler, Lena
Citation: Bjorneras, Johannes; Graslund, Astrid; Maler, Lena. "Membrane interaction of disease-related dynorphin a variants." Biochemistry 52, 4157-4167 (2013).
Assembly members:
R6W-DynA, polymer, 17 residues, 2177 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
R6W-DynA: YGGFLWRIRPKLKWDNQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 137 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | R6W-DynA | 1 |
Entities:
Entity 1, R6W-DynA 17 residues - 2177 Da.
| 1 | TYR | GLY | GLY | PHE | LEU | TRP | ARG | ILE | ARG | PRO | ||||
| 2 | LYS | LEU | LYS | TRP | ASP | ASN | GLN |
Samples:
sample_1: R6W-DynA 1 mM; H2O 90%; D2O 10%; sodium phosphate 50 mM
sample_2: R6W-DynA 1 mM; H2O 90%; D2O 10%; sodium phosphate 50 mM; DMPC, [U-2H], 35 mM; DHPC, [U-2H], 115 mM
sample_3: R6W-DynA 1 mM; H2O 90%; D2O 10%; sodium phosphate 50 mM; DMPG, [U-2H], 7 mM; DMPC, [U-2H], 28 mM; DHPC, [U-2H], 115 mM
sample_conditions_1: ionic strength: 0.2 M; pH: 5.7; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_3 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_3 | isotropic | sample_conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 500 MHz