BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19155

Title: NMR Structure of CbpAN from Streptococcus pneumoniae

Deposition date: 2013-04-10 Original release date: 2014-04-14

Authors: Liu, Aizhuo; Yan, Honggao; Achila, David; Martinez-Hackert, Erik; Li, Yue; Banerjee, Rahul

Citation: Liu, Aizhuo; Achila, David; Banerjee, Rahul; Martinez-Hackert, Erik; Li, Yue; Yan, Honggao. "Host Specification of Factor H Binding by Streptococcus pneumoniae"  Nature ., .-..

Assembly members:
entity, polymer, 85 residues, 9522.928 Da.

Natural source:   Common Name: firmicutes   Taxonomy ID: 1313   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus pneumoniae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: GHMLDSERDKARKEVEEYVK KIVGESYAKSTKKRHTITVA LVNELNNIKNEYLNKIVEST SESQLQILMMESRSKVDEAV SKFEK

Data sets:
Data typeCount
13C chemical shifts356
15N chemical shifts87
1H chemical shifts602

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CbpAN1

Entities:

Entity 1, CbpAN 85 residues - 9522.928 Da.

1   GLYHISMETLEUASPSERGLUARGASPLYS
2   ALAARGLYSGLUVALGLUGLUTYRVALLYS
3   LYSILEVALGLYGLUSERTYRALALYSSER
4   THRLYSLYSARGHISTHRILETHRVALALA
5   LEUVALASNGLULEUASNASNILELYSASN
6   GLUTYRLEUASNLYSILEVALGLUSERTHR
7   SERGLUSERGLNLEUGLNILELEUMETMET
8   GLUSERARGSERLYSVALASPGLUALAVAL
9   SERLYSPHEGLULYS

Samples:

sample_1: DSS 50 uM; sodium azide 100 uM; sodium phosphate 50 mM; sodium chloride 50 mM; H20 95%; D20 5%; CbpAN_protein, 15N/13C-labeled, 1 mM

sample_2: DSS 50 uM; sodium azide 100 uM; sodium phosphate 50 mM; sodium chloride 50 mM; D20 100%; CbpAN_protein, 15N/13C-labeled, 1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

PDB
EMBL CEV61387 CEW81978 CEW91983 CEW94993 CEY00327
GB AAF13459 AAF73792 AAF73796 AAF73807 AAF73821
REF WP_000458186 WP_000458187 WP_000458188 WP_000458189 WP_000458190

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts