BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19170

Title: solution structure of the calmodulin-binding domain of plant calcium-ATPase ACA8

Deposition date: 2013-04-16 Original release date: 2014-04-14

Authors: Jamshidiha, Mostafa; Ishida, Hiroaki; Gifford, Jessica; Vogel, Hans

Citation: Jamshidiha, Mostafa; Ishida, Hiroaki; Gifford, Jessica; Vogel, Hans. "Structural characterization of the interaction between a plant calmodulin and three distinct calcium-ATPase pumps"  Not known ., .-..

Assembly members:
ACA8, polymer, 25 residues, 3110.716 Da.

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: chemical synthesis   Host organism: Arabidopsis thaliana

Entity Sequences (FASTA):
ACA8: RLQQWRKAALVLNASRRFRY TLDLK

Data sets:
Data typeCount
1H chemical shifts206

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ACA81

Entities:

Entity 1, ACA8 25 residues - 3110.716 Da.

1   ARGLEUGLNGLNTRPARGLYSALAALALEU
2   VALLEUASNALASERARGARGPHEARGTYR
3   THRLEUASPLEULYS

Samples:

sample_1: D2O, [U-100% 2H], 10%; DSS, [U-100% 2H], 5 mM; H2O 60%; TFE, [U-100% 2H], 30%; DTT, [U-100% 2H], 10 mM; trifluoroethanol 40%; ACA8 mM

sample_conditions_1: ionic strength: 0 M; pH: 4.2; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ BAA97361 BAE99029 BAH20100 BAJ34212
EMBL CAB96189 CDY20693 CDY32902 CDY38264 CDY53981
GB AAL47426 AAN60274 AED96847 AED96848 EFH42443
REF NP_200521 NP_851200 XP_002866184 XP_006281735 XP_006401244
SP Q9LF79