BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19174

Title: Structure of the Regulatory Domain of Human Brain Carnitine Palmitoyltransferase 1   PubMed: 24037959

Deposition date: 2013-04-18 Original release date: 2013-09-10

Authors: Samanta, Soma; Situ, Alan; Ulmer, Tobias

Citation: Samanta, Soma; Situ, Alan; Ulmer, Tobias. "Structural characterization of the regulatory domain of brain carnitine palmitoyltransferase 1."  Biopolymers 101, 398-405 (2014).

Assembly members:
Palmitoyltransferase_1, polymer, 50 residues, 5711.461 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Palmitoyltransferase_1: MAEAHQAVGFRPSLTSDGAE VELSAPVLQEIYLSGLRSWK RHLSRFWNDF

Data sets:
Data typeCount
13C chemical shifts91
15N chemical shifts47
1H chemical shifts47

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Palmitoyltransferase_11

Entities:

Entity 1, Palmitoyltransferase_1 50 residues - 5711.461 Da.

1   METALAGLUALAHISGLNALAVALGLYPHE
2   ARGPROSERLEUTHRSERASPGLYALAGLU
3   VALGLULEUSERALAPROVALLEUGLNGLU
4   ILETYRLEUSERGLYLEUARGSERTRPLYS
5   ARGHISLEUSERARGPHETRPASNASPPHE

Samples:

sample_1: Palmitoyltransferase_1, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 mM; dodecyltrimethylammonium chloride 150 mM; MES 25 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.175 M; pH: 5.6; pressure: 1 atm; temperature: 308.2 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
Quant J correlationsample_1isotropicsample_conditions_1
Quant J correlationsample_1anisotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAF82402 BAF82781
EMBL CAH90871
GB AAH29104 AAL99615 AAQ14875 ABM82478 ABM85665
REF NP_001125498 NP_001129524 NP_001186681 NP_001186682 NP_689572
SP Q8TCG5

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts