BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19217

Title: SmTSP2EC2   PubMed: 24429291

Deposition date: 2013-05-02 Original release date: 2014-01-21

Authors: Mulvenna, Jason; Jia, Xinying

Citation: Jia, Xinying; Schulte, Leigh; Loukas, Alex; Pickering, Darren; Pearson, Mark; Mobli, Mehdi; Jones, Alun; Rosengren, Karl; Daly, Norelle; Gobert, Geoffrey; Jones, Malcolm; Craik, David; Mulvenna, Jason. "Solution Structure, Membrane Interactions, and Protein Binding Partners of the Tetraspanin Sm-TSP-2, a Vaccine Antigen from the Human Blood Fluke Schistosoma mansoni."  J. Biol. Chem. 289, 7151-7163 (2014).

Assembly members:
SmTSP2EC2, polymer, 81 residues, 9091.423 Da.

Natural source:   Common Name: Flatworm   Taxonomy ID: 6183   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Schistosoma mansoni

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
SmTSP2EC2: GSNEKPKVKKHITSALKKLV DKYRNDEHVRKVFDEIQQKL HCCGADSPKDYGENPPTSCS KDGVQFTEGCIKKVSDLSKA H

Data sets:
Data typeCount
13C chemical shifts274
15N chemical shifts82
1H chemical shifts556

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SmTSP2EC21

Entities:

Entity 1, SmTSP2EC2 81 residues - 9091.423 Da.

1   GLYSERASNGLULYSPROLYSVALLYSLYS
2   HISILETHRSERALALEULYSLYSLEUVAL
3   ASPLYSTYRARGASNASPGLUHISVALARG
4   LYSVALPHEASPGLUILEGLNGLNLYSLEU
5   HISCYSCYSGLYALAASPSERPROLYSASP
6   TYRGLYGLUASNPROPROTHRSERCYSSER
7   LYSASPGLYVALGLNPHETHRGLUGLYCYS
8   ILELYSLYSVALSERASPLEUSERLYSALA
9   HIS

Samples:

sample_1: SmTSP2EC2, [U-100% 13C; U-100% 15N], 1.4 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
4D HCC(CO)NH-TOCSYsample_1isotropicsample_conditions_1
2D (H)CB-(CGCCTOCSY)-Harsample_1isotropicsample_conditions_1
Tyr-selective 2D (H)CB-(CGCCTOCSY)-Harsample_1isotropicsample_conditions_1
Phe-selective 2D (H)CB-(CGCCTOCSY)-Harsample_1isotropicsample_conditions_1

Software:

CYANA, Bruker Biospin, CCPN, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, collection, peak picking, processing, structure solution

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

PDB
GB AAN17276

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts