BMRB Entry 19247
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19247
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone (1H,15N, CA, CB) assignments of Populus nigra Plastocyanin (Zn)
Deposition date: 2013-05-17 Original release date: 2013-06-04
Authors: Guan, Jia-Ying; Ubbink, Marcellus
Citation: Guan, Jia-Ying; Foester, Johannes; Drijfhout, Jan Wouter; Timmer, Monika; Blok, Anneloes; Ullmann, Matthias; Ubbink, Marcellus. "Dynamics between plastocyanin and tetralysine peptides" Not known ., .-..
Assembly members:
plastocyanin, polymer, 101 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Lombardy poplar Taxonomy ID: 3691 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Populus nigra
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
plastocyanin: MGIDVLLGADDGSLAFVPSE
FSISPGEKIVFKNNAGFPHN
IVFDEDSIPSGVDASKISMS
EEDLLNAKGETFEVALSNKG
EYSFYCSPHQGAGMVGKVTV
N
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 226 |
15N chemical shifts | 114 |
1H chemical shifts | 118 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | plastocyanin | 1 |
2 | zinc | 2 |
Entities:
Entity 1, plastocyanin 101 residues - Formula weight is not available
1.The first two residues in the sequence (MG) are not included in the assignment data. The residue numbers in the assignment correspond to the sequence starting from the third residue (I). 2.N-terminal methionine was partially processed and therefore some residues near N-terminus in space have double resonances.
1 | MET | GLY | ILE | ASP | VAL | LEU | LEU | GLY | ALA | ASP | ||||
2 | ASP | GLY | SER | LEU | ALA | PHE | VAL | PRO | SER | GLU | ||||
3 | PHE | SER | ILE | SER | PRO | GLY | GLU | LYS | ILE | VAL | ||||
4 | PHE | LYS | ASN | ASN | ALA | GLY | PHE | PRO | HIS | ASN | ||||
5 | ILE | VAL | PHE | ASP | GLU | ASP | SER | ILE | PRO | SER | ||||
6 | GLY | VAL | ASP | ALA | SER | LYS | ILE | SER | MET | SER | ||||
7 | GLU | GLU | ASP | LEU | LEU | ASN | ALA | LYS | GLY | GLU | ||||
8 | THR | PHE | GLU | VAL | ALA | LEU | SER | ASN | LYS | GLY | ||||
9 | GLU | TYR | SER | PHE | TYR | CYS | SER | PRO | HIS | GLN | ||||
10 | GLY | ALA | GLY | MET | VAL | GLY | LYS | VAL | THR | VAL | ||||
11 | ASN |
Entity 2, zinc - Zn - 65.409 Da.
1 | ZN |
Samples:
sample_1: plastocyanin, [U-13C; U-15N], 0.25 ± 0.01 mM; sodium phosphate 10 mM
sample_conditions_1: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 300 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
PDB | |
BMRB | 4019 |
EMBL | CAA90564 |
GB | ABK96770 EEE94750 |
REF | XP_002307754 XP_011003094 |
SP | P00299 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts