BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19253

Title: Backbone and Cbeta assignments for tau K18 in free and SDS micelle-bound states   PubMed: 23740819

Deposition date: 2013-05-21 Original release date: 2014-02-13

Authors: Eliezer, David; Barr, Patrick

Citation: Barre, Patrick; Eliezer, David. "Structural transitions in tau k18 on micelle binding suggest a hierarchy in the efficacy of individual microtubule-binding repeats in filament nucleation."  Protein Sci. 22, 1037-1048 (2013).

Assembly members:
tau_K18, polymer, 129 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
tau_K18: QTAPVPMPDLKNVKSKIGST ENLKHQPGGGKVQIINKKLD LSNVQSKCGSKDNIKHVPGG GSVQIVYKPVDLSKVTSKAG SLGNIHHKPGGGQVEVKSEK LDFKDRVQSKIGSLDNITHV PGGGNKKIE

Data sets:
Data typeCount
13C chemical shifts740
15N chemical shifts230
1H chemical shifts230

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tau_K181

Entities:

Entity 1, tau_K18 129 residues - Formula weight is not available

1   GLNTHRALAPROVALPROMETPROASPLEU
2   LYSASNVALLYSSERLYSILEGLYSERTHR
3   GLUASNLEULYSHISGLNPROGLYGLYGLY
4   LYSVALGLNILEILEASNLYSLYSLEUASP
5   LEUSERASNVALGLNSERLYSCYSGLYSER
6   LYSASPASNILELYSHISVALPROGLYGLY
7   GLYSERVALGLNILEVALTYRLYSPROVAL
8   ASPLEUSERLYSVALTHRSERLYSALAGLY
9   SERLEUGLYASNILEHISHISLYSPROGLY
10   GLYGLYGLNVALGLUVALLYSSERGLULYS
11   LEUASPPHELYSASPARGVALGLNSERLYS
12   ILEGLYSERLEUASPASNILETHRHISVAL
13   PROGLYGLYGLYASNLYSLYSILEGLU

Samples:

sample_1: tau_K18, [U-100% 13C; U-100% 15N], 400 – 800 uM; H20 90%; D20 10%

sample_2: tau K18, [U-98% 13C; U-98% 15N], 400 – 800 uM; SDS 100 mM; H20 90%; D20 10%

sample_conditions_1: ionic strength: 160 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

sample_conditions_2: ionic strength: 160 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_2
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_2
3D HNCOsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_2
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCACOsample_2isotropicsample_conditions_2

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Bruker DRX 700 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 17920
DBJ BAE28038
EMBL CAA32636 CAA55889
GB AAA30770 AAA42204 AAA51609 AAA58343 AAA58344
REF NP_001009068 NP_001033698 NP_001104271 NP_001116538 NP_001116539
SP O02828 P10636 P10637 P19332 P29172
TPE CAG26750
TPG DAA18338

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts