BMRB Entry 19253
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19253
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Title: Backbone and Cbeta assignments for tau K18 in free and SDS micelle-bound states PubMed: 23740819
Deposition date: 2013-05-21 Original release date: 2014-02-13
Authors: Eliezer, David; Barr, Patrick
Citation: Barre, Patrick; Eliezer, David. "Structural transitions in tau k18 on micelle binding suggest a hierarchy in the efficacy of individual microtubule-binding repeats in filament nucleation." Protein Sci. 22, 1037-1048 (2013).
Assembly members:
tau_K18, polymer, 129 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
tau_K18: QTAPVPMPDLKNVKSKIGST
ENLKHQPGGGKVQIINKKLD
LSNVQSKCGSKDNIKHVPGG
GSVQIVYKPVDLSKVTSKAG
SLGNIHHKPGGGQVEVKSEK
LDFKDRVQSKIGSLDNITHV
PGGGNKKIE
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 740 |
15N chemical shifts | 230 |
1H chemical shifts | 230 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | tau_K18 | 1 |
Entities:
Entity 1, tau_K18 129 residues - Formula weight is not available
1 | GLN | THR | ALA | PRO | VAL | PRO | MET | PRO | ASP | LEU | ||||
2 | LYS | ASN | VAL | LYS | SER | LYS | ILE | GLY | SER | THR | ||||
3 | GLU | ASN | LEU | LYS | HIS | GLN | PRO | GLY | GLY | GLY | ||||
4 | LYS | VAL | GLN | ILE | ILE | ASN | LYS | LYS | LEU | ASP | ||||
5 | LEU | SER | ASN | VAL | GLN | SER | LYS | CYS | GLY | SER | ||||
6 | LYS | ASP | ASN | ILE | LYS | HIS | VAL | PRO | GLY | GLY | ||||
7 | GLY | SER | VAL | GLN | ILE | VAL | TYR | LYS | PRO | VAL | ||||
8 | ASP | LEU | SER | LYS | VAL | THR | SER | LYS | ALA | GLY | ||||
9 | SER | LEU | GLY | ASN | ILE | HIS | HIS | LYS | PRO | GLY | ||||
10 | GLY | GLY | GLN | VAL | GLU | VAL | LYS | SER | GLU | LYS | ||||
11 | LEU | ASP | PHE | LYS | ASP | ARG | VAL | GLN | SER | LYS | ||||
12 | ILE | GLY | SER | LEU | ASP | ASN | ILE | THR | HIS | VAL | ||||
13 | PRO | GLY | GLY | GLY | ASN | LYS | LYS | ILE | GLU |
Samples:
sample_1: tau_K18, [U-100% 13C; U-100% 15N], 400 800 uM; H20 90%; D20 10%
sample_2: tau K18, [U-98% 13C; U-98% 15N], 400 800 uM; SDS 100 mM; H20 90%; D20 10%
sample_conditions_1: ionic strength: 160 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K
sample_conditions_2: ionic strength: 160 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_2 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_2 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_2 | isotropic | sample_conditions_2 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_2 | isotropic | sample_conditions_2 |
3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACO | sample_2 | isotropic | sample_conditions_2 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - data analysis
NMR spectrometers:
- Bruker DRX 700 MHz
- Bruker DRX 800 MHz
Related Database Links:
BMRB | 17920 |
DBJ | BAE28038 |
EMBL | CAA32636 CAA55889 |
GB | AAA30770 AAA42204 AAA51609 AAA58343 AAA58344 |
REF | NP_001009068 NP_001033698 NP_001104271 NP_001116538 NP_001116539 |
SP | O02828 P10636 P10637 P19332 P29172 |
TPE | CAG26750 |
TPG | DAA18338 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts