BMRB Entry 19267
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19267
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Title: Backbone Assignment of Outer Envelope Protein 16kDa PubMed: 24205117
Deposition date: 2013-05-26 Original release date: 2014-02-13
Authors: Zook, James; Molugu, Trivikram; Jacobsen, Neil; Lin, Guangxin; Brown, Michael; Fromme, Petra
Citation: Zook, James; Molugu, Trivikram; Jacobsen, Neil; Lin, Guangxin; Soll, Jurgen; Cherry, Brian; Brown, Michael; Fromme, Petra. "High-resolution NMR reveals secondary structure and folding of amino acid transporter from outer chloroplast membrane." PLoS ONE 8, e78116-e78116 (2013).
Assembly members:
OEP16, polymer, 154 residues, Formula weight is not available
Natural source: Common Name: Eudicots Taxonomy ID: 3888 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Pisum sativum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
OEP16: MPRSSFSGSLSSPKLDVVID
MGNPFLNLTVDGFLKIGAVA
ATRSVAEDTFHIIRKGSISS
NDFEKSLKKMCKEGAYWGAI
AGVYVGMEYGVERIRGTRDW
KNAMFGGAVTGALVSAASNN
KKDKIAVDAITGAAIATAAE
FINYLTLEHHHHHH
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 443 |
15N chemical shifts | 150 |
1H chemical shifts | 271 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | OEP16 | 1 |
Entities:
Entity 1, OEP16 154 residues - Formula weight is not available
This is a membrane protein with a non-native, C-terminal 6x Histidine tag
1 | MET | PRO | ARG | SER | SER | PHE | SER | GLY | SER | LEU | ||||
2 | SER | SER | PRO | LYS | LEU | ASP | VAL | VAL | ILE | ASP | ||||
3 | MET | GLY | ASN | PRO | PHE | LEU | ASN | LEU | THR | VAL | ||||
4 | ASP | GLY | PHE | LEU | LYS | ILE | GLY | ALA | VAL | ALA | ||||
5 | ALA | THR | ARG | SER | VAL | ALA | GLU | ASP | THR | PHE | ||||
6 | HIS | ILE | ILE | ARG | LYS | GLY | SER | ILE | SER | SER | ||||
7 | ASN | ASP | PHE | GLU | LYS | SER | LEU | LYS | LYS | MET | ||||
8 | CYS | LYS | GLU | GLY | ALA | TYR | TRP | GLY | ALA | ILE | ||||
9 | ALA | GLY | VAL | TYR | VAL | GLY | MET | GLU | TYR | GLY | ||||
10 | VAL | GLU | ARG | ILE | ARG | GLY | THR | ARG | ASP | TRP | ||||
11 | LYS | ASN | ALA | MET | PHE | GLY | GLY | ALA | VAL | THR | ||||
12 | GLY | ALA | LEU | VAL | SER | ALA | ALA | SER | ASN | ASN | ||||
13 | LYS | LYS | ASP | LYS | ILE | ALA | VAL | ASP | ALA | ILE | ||||
14 | THR | GLY | ALA | ALA | ILE | ALA | THR | ALA | ALA | GLU | ||||
15 | PHE | ILE | ASN | TYR | LEU | THR | LEU | GLU | HIS | HIS | ||||
16 | HIS | HIS | HIS | HIS |
Samples:
sample_1: sodium chloride 100 mM; sodium phosphate 20 mM; EDTA 1 mM; beta-mercaptoethanol 1 mM; glycerol 10 v/v; SDS 0.4 w/v; sodium azide 0.02 w/v; OEP16, [U-99% 15N], 1.5 mM; H2O 90%; D2O 10%
sample_2: sodium chloride 100 mM; sodium phosphate 20 mM; EDTA 1 mM; beta-mercaptoethanol 1 mM; glycerol 10 v/v; SDS 0.4 w/v; sodium azide 0.02 w/v; OEP16, [U-99% 13C; U-99% 15N; U-80% 2H], 1.0 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.14 M; pH: 6.5; pressure: 1 atm; temperature: 310 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
3D HNCO | sample_2 | isotropic | sample_conditions_1 |
3D HNCA | sample_2 | isotropic | sample_conditions_1 |
3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_2 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_2 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.113, Goddard - chemical shift assignment, chemical shift calculation, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
VNMRJ, Varian - collection
NMR spectrometers:
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts