BMRB Entry 19270
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19270
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Title: Backbone assignment of the Sigma-1 receptor chaperone domain PubMed: 23760505
Deposition date: 2013-05-27 Original release date: 2014-02-13
Authors: Ortega-Roldan, Jose L.; Schnell, Jason
Citation: Ortega-Roldan, Jose Luis; Ossa, Felipe; Schnell, Jason. "Characterization of the human sigma-1 receptor chaperone domain structure and binding immunoglobulin protein (BiP) interactions." J. Biol. Chem. 288, 21448-21457 (2013).
Assembly members:
S1ch, polymer, 129 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
S1ch: MNHKVHHHHHHIEGRHMGSR
GHSGRYWAEISDTIISGTFH
QWREGTTKSEVFYPGETVVH
GPGEATAVEWGPNTWMVEYG
RGVIPSTLAFALADTVFSTQ
DFLTLFYTLRSYARGLRLEL
TTYLFGQDP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 297 |
| 15N chemical shifts | 105 |
| 1H chemical shifts | 214 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | S1ch | 1 |
Entities:
Entity 1, S1ch 129 residues - Formula weight is not available
| 1 | MET | ASN | HIS | LYS | VAL | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | HIS | ILE | GLU | GLY | ARG | HIS | MET | GLY | SER | ARG | ||||
| 3 | GLY | HIS | SER | GLY | ARG | TYR | TRP | ALA | GLU | ILE | ||||
| 4 | SER | ASP | THR | ILE | ILE | SER | GLY | THR | PHE | HIS | ||||
| 5 | GLN | TRP | ARG | GLU | GLY | THR | THR | LYS | SER | GLU | ||||
| 6 | VAL | PHE | TYR | PRO | GLY | GLU | THR | VAL | VAL | HIS | ||||
| 7 | GLY | PRO | GLY | GLU | ALA | THR | ALA | VAL | GLU | TRP | ||||
| 8 | GLY | PRO | ASN | THR | TRP | MET | VAL | GLU | TYR | GLY | ||||
| 9 | ARG | GLY | VAL | ILE | PRO | SER | THR | LEU | ALA | PHE | ||||
| 10 | ALA | LEU | ALA | ASP | THR | VAL | PHE | SER | THR | GLN | ||||
| 11 | ASP | PHE | LEU | THR | LEU | PHE | TYR | THR | LEU | ARG | ||||
| 12 | SER | TYR | ALA | ARG | GLY | LEU | ARG | LEU | GLU | LEU | ||||
| 13 | THR | THR | TYR | LEU | PHE | GLY | GLN | ASP | PRO |
Samples:
sample_1: S1ch, [U-100% 13C; U-100% 15N; U-80% 2H], 0.27 mM; S1ch 95%; S1ch 5%
sample_2: S1ch, [U-100% 13C; U-100% 15N], 0.27 mM; S1ch 95%; S1ch 5%
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 500 MHz
Related Database Links:
| EMBL | CAG33356 |
| GB | AAB50402 AAB51238 AAC04507 AAH04899 ABM83807 |
| REF | NP_001244806 NP_001269135 NP_001269136 NP_005857 XP_002743083 |
| SP | Q99720 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts