BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19294

Title: DNA-binding domain of T. brucei telomeric protein tbTRF

Deposition date: 2013-06-09 Original release date: 2014-07-14

Authors: LI, Xiaohua; YE, Fei; ZHANG, Mingjie; ZHAO, Yanxiang

Citation: LI, Xiaohua; YE, Fei; ZHANG, Mingjie; ZHAO, Yanxiang. "DNA-binding domain of T. brucei telomeric protein tbTRF"  Not known ., .-..

Assembly members:
entity, polymer, 106 residues, 12054.533 Da.

Natural source:   Common Name: dog   Taxonomy ID: 9615   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Canis familiaris

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: GPGSLSNFANVGVGTSSGKQ KRYKFSASEDEAIIKGLARF TKGQQRFQQIYYAYRSVWHP ARTVSQLYDHWRGTLRYKVI QQQGYRGKNSVAARSPEKAS NMENNE

Data sets:
Data typeCount
15N chemical shifts98
1H chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA-binding domain of T. brucei telomeric protein tbTRF1

Entities:

Entity 1, DNA-binding domain of T. brucei telomeric protein tbTRF 106 residues - 12054.533 Da.

1   GLYPROGLYSERLEUSERASNPHEALAASN
2   VALGLYVALGLYTHRSERSERGLYLYSGLN
3   LYSARGTYRLYSPHESERALASERGLUASP
4   GLUALAILEILELYSGLYLEUALAARGPHE
5   THRLYSGLYGLNGLNARGPHEGLNGLNILE
6   TYRTYRALATYRARGSERVALTRPHISPRO
7   ALAARGTHRVALSERGLNLEUTYRASPHIS
8   TRPARGGLYTHRLEUARGTYRLYSVALILE
9   GLNGLNGLNGLYTYRARGGLYLYSASNSER
10   VALALAALAARGSERPROGLULYSALASER
11   ASNMETGLUASNASNGLU

Samples:

sample_1: DNA-binding domain of T. brucei telomeric protein tbTRF mM; potassium phosphate 100 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 5.6; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB
EMBL CBH16451
GB AAX86992 EAN78659
REF XP_011778715 XP_823487

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts