BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19476

Title: NMR SOLUTION STRUCTURE OF ALPHA CONOTOXIN LO1A FROM Conus longurionis   PubMed: 24567324

Deposition date: 2013-09-01 Original release date: 2014-03-03

Authors: Maiti, M.; Lescrinier, E.; Herdewijn, P.; Lebbe, E.; Peigneur, S.; D'Souza, L.; Tytgat, J.

Citation: Lebbe, Eline; Peigneur, Steve; Maiti, Mohitosh; Devi, Prabha; Ravichandran, Samuthirapandian; Lescrinier, Eveline; Ulens, Chris; Waelkens, Etienne; Herdewijn, Lisette; Tytgat, Piet. "Structure-Function Elucidation of a New -Conotoxin, Lo1a, from Conus longurionis."  J. Biol. Chem. 289, 9573-9583 (2014).

Assembly members:
CONOTOXIN_LO1A, polymer, 18 residues, 1937.117 Da.

Natural source:   Common Name: Conus longurionis   Taxonomy ID: 1077918   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus longurionis

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
CONOTOXIN_LO1A: EGCCSNPACRTNHPEVCD

Data sets:
Data typeCount
13C chemical shifts48
1H chemical shifts103

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CONOTOXIN LO1A1

Entities:

Entity 1, CONOTOXIN LO1A 18 residues - 1937.117 Da.

1   GLUGLYCYSCYSSERASNPROALACYSARG
2   THRASNHISPROGLUVALCYSASP

Samples:

sample: CONOTOXIN LO1A 2.6 mM

sample_conditions_1: pH: 5.9; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsampleisotropicsample_conditions_1
2D 1H-1H NOESYsampleisotropicsample_conditions_1
2D 1H-1H TOCSYsampleisotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsampleisotropicsample_conditions_1
2D 1H-13C HSQC aromaticsampleisotropicsample_conditions_1

Software:

X-PLOR NIH v2.25, SCHWIETERS, KUSZEWSKI, TJ - refinement

TOPSPIN v2.1, Bruker - processing

NMR spectrometers:

  • BRUKER AVANCE 600 MHz

Related Database Links:

PDB
SP X1WB75