BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19535

Title: SGTX-Sf1a

Deposition date: 2013-10-04 Original release date: 2014-10-13

Authors: Mobli, Mehdi; Bende, Niraj; King, Glenn

Citation: Mobli, Mehdi; Bende, Niraj; King, Glenn; Volker, Herzig. "Structure of SGTX1-SF1A"  Not known ., .-..

Assembly members:
SF1A, polymer, 47 residues, 5073.909 Da.

Natural source:   Common Name: Tube web spider   Taxonomy ID: 31925   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Segestria florentina

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
SF1A: SKECMTDGTVCYIHNHNDCC GSCLCSNGPIARPWEMMVGN CMCGPKA

Data sets:
Data typeCount
13C chemical shifts182
15N chemical shifts49
1H chemical shifts291

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SGTX-Sf1a1

Entities:

Entity 1, SGTX-Sf1a 47 residues - 5073.909 Da.

1   SERLYSGLUCYSMETTHRASPGLYTHRVAL
2   CYSTYRILEHISASNHISASNASPCYSCYS
3   GLYSERCYSLEUCYSSERASNGLYPROILE
4   ALAARGPROTRPGLUMETMETVALGLYASN
5   CYSMETCYSGLYPROLYSALA

Samples:

sample_1: SF1A, [U-99% 13C; U-99% 15N], 0.42 mM; D2O, [U-100% 2H], 5%; Sodium citrate 20 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 20 mM; pH: 3.5; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
4D HC(CO)NHsample_1isotropicsample_conditions_1
2D (HB)CB(CGCC)H(ar)sample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

Rownald_NMR_Toolkit vv3, Jeffrey C. Hoch - processing

CYANA v3, Guntert, Mumenthaler and Wuthrich - structure solution

TOPSPIN v3, Bruker Biospin - collection

TALOS v+, Cornilescu, Delaglio and Bax - structure solution

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

PDB
SP P61095

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts