BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19617

Title: Solution structure of the Big domain from Leptospira interrogans

Deposition date: 2013-11-17 Original release date: 2013-12-09

Authors: Mei, Song

Citation: Mei, Song. "Solution structure of the Big domain from Leptospira interrogans"  Not known ., .-..

Assembly members:
entity, polymer, 96 residues, 9481.341 Da.

Natural source:   Common Name: spirochetes   Taxonomy ID: 173   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Leptospira interrogans

Experimental source:   Production method: recombinant technology   Host organism: Leptospira interrogans

Entity Sequences (FASTA):
entity: MPAALVSISVSPTNSTVAKG LQENFKATGIFTDNSNSDIT DQVTWDSSNTDILSISNASD SHGLASTLNQGNVKVTASIG GIQGSTDFKVTQALEH

Data sets:
Data typeCount
13C chemical shifts248
15N chemical shifts92
1H chemical shifts457

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Big domain from Leptospira interrogans1

Entities:

Entity 1, Big domain from Leptospira interrogans 96 residues - 9481.341 Da.

1   METPROALAALALEUVALSERILESERVAL
2   SERPROTHRASNSERTHRVALALALYSGLY
3   LEUGLNGLUASNPHELYSALATHRGLYILE
4   PHETHRASPASNSERASNSERASPILETHR
5   ASPGLNVALTHRTRPASPSERSERASNTHR
6   ASPILELEUSERILESERASNALASERASP
7   SERHISGLYLEUALASERTHRLEUASNGLN
8   GLYASNVALLYSVALTHRALASERILEGLY
9   GLYILEGLNGLYSERTHRASPPHELYSVAL
10   THRGLNALALEUGLUHIS

Samples:

sample_1: ammonium chloride, [U-99% 15N], 0.5 g/L; glucose, [U-95% 13C], 2.5 g/L; H2O 90%; D2O 10%; entity, [U-13C; U-15N], 0.5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - structure solution

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 25600 25819
PDB

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts