BMRB Entry 19699
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19699
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Title: Structure of the m04/gp34 mouse Cytomegalovirus Immunoevasin core domain
Deposition date: 2013-12-21 Original release date: 2014-07-14
Authors: Sgourakis, Nikolaos; Natarajan, Kannan; Margulies, David; Bax, Ad
Citation: Sgourakis, Nikolaos; Natarajan, Kannan; Margulies, David; Bax, Ad. "The murine Cytomegalovirus gp34/m04 protein contains a core domain with novel structure revealed by solution NMR" Not known ., .-..
Assembly members:
entity, polymer, 156 residues, 17494.967 Da.
Natural source: Common Name: Maize chlorotic mottle virus Taxonomy ID: 12138 Superkingdom: Virus Kingdom: not available Genus/species: Machlomovirus Maize chlorotic mottle virus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: RHSLGCYFKGINPTKVPSSD
PRTVLKCTLPDVKVNASWTL
EWVVVNLHTSVDVTSYYESS
PNSEPRFLRAILNFTPMHGL
RTKNLLKVKDGFQVDNSTDN
GNGGNLYVYPNATTGSADSV
RCRLRMCPWTSNSKMTAPDE
EMLRKMSEVLNLPNYG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 411 |
15N chemical shifts | 143 |
1H chemical shifts | 143 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | gp34/m04 | 1 |
Entities:
Entity 1, gp34/m04 156 residues - 17494.967 Da.
1 | ARG | HIS | SER | LEU | GLY | CYS | TYR | PHE | LYS | GLY | ||||
2 | ILE | ASN | PRO | THR | LYS | VAL | PRO | SER | SER | ASP | ||||
3 | PRO | ARG | THR | VAL | LEU | LYS | CYS | THR | LEU | PRO | ||||
4 | ASP | VAL | LYS | VAL | ASN | ALA | SER | TRP | THR | LEU | ||||
5 | GLU | TRP | VAL | VAL | VAL | ASN | LEU | HIS | THR | SER | ||||
6 | VAL | ASP | VAL | THR | SER | TYR | TYR | GLU | SER | SER | ||||
7 | PRO | ASN | SER | GLU | PRO | ARG | PHE | LEU | ARG | ALA | ||||
8 | ILE | LEU | ASN | PHE | THR | PRO | MET | HIS | GLY | LEU | ||||
9 | ARG | THR | LYS | ASN | LEU | LEU | LYS | VAL | LYS | ASP | ||||
10 | GLY | PHE | GLN | VAL | ASP | ASN | SER | THR | ASP | ASN | ||||
11 | GLY | ASN | GLY | GLY | ASN | LEU | TYR | VAL | TYR | PRO | ||||
12 | ASN | ALA | THR | THR | GLY | SER | ALA | ASP | SER | VAL | ||||
13 | ARG | CYS | ARG | LEU | ARG | MET | CYS | PRO | TRP | THR | ||||
14 | SER | ASN | SER | LYS | MET | THR | ALA | PRO | ASP | GLU | ||||
15 | GLU | MET | LEU | ARG | LYS | MET | SER | GLU | VAL | LEU | ||||
16 | ASN | LEU | PRO | ASN | TYR | GLY |
Samples:
3D: sodium phosphate 20mM mM; D2O, [U-99% 2H], 5%; sodium chloride 50 mM; gp34/m04, [U-13C; U-15N; U-2H], 0.2 0.5 mM; H2O 95
ILV_CC: sodium phosphate 20mM mM; D2O, [U-99% 2H], 5%; sodium chloride 50 mM; gp34/m04, [U-13C; U-15N; U-2H; ILVmethyl-1H], 0.2 0.5 mM; H2O 95
GEL: sodium phosphate 20mM mM; D2O, [U-99% 2H], 5%; sodium chloride 50 mM; gp34/m04, [U-15N; U-2H], 0.2 0.5 mM; Ac/Bis-Ac 5.5%; DADMAC 20%; H2O 95
Pf1: sodium phosphate 20mM mM; D2O, [U-99% 2H], 5%; sodium chloride 200 mM; gp34/m04, [U-15N; U-2H], 0.2 0.5 mM; Pf1 phage 7.5%; H2O 95
ILV_NOE: sodium phosphate 20mM mM; D2O, [U-99% 2H], 5%; sodium chloride 50 mM; gp34/m04, [ILVmethyl-13C; U-15N; U-2H; ILVmethyl-1H], 0.2 0.5 mM; H2O 95
2D: sodium phosphate 20mM mM; D2O, [U-99% 2H], 5%; sodium chloride 50 mM; gp34/m04, [U-15N; U-2H], 0.2 0.5 mM; H2O 95
sample_conditions_1: ionic strength: 0.110 M; pH: 6.5; pressure: 1 atm; temperature: 285 K
sample_conditions_2: ionic strength: 0.260 M; pH: 6.5; pressure: 1 atm; temperature: 285 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N TROSY-HSQC | 3D | isotropic | sample_conditions_1 |
3D HNCA | 3D | isotropic | sample_conditions_1 |
3D HNCACB | 3D | isotropic | sample_conditions_1 |
3D HNCO | 3D | isotropic | sample_conditions_1 |
3D HN(CA)CO | 3D | isotropic | sample_conditions_1 |
2D 1H-15N ARTSY | Pf1 | anisotropic | sample_conditions_2 |
2D 1H-15N ARTSY | GEL | anisotropic | sample_conditions_1 |
3D 1H-13C NOESY aliphatic (HCH) | ILV_NOE | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aliphatic (CCH) | ILV_NOE | isotropic | sample_conditions_1 |
2D 1H-13C HMQC methyl | ILV_NOE | isotropic | sample_conditions_1 |
3D 1H-15N NOESY (HCH) | ILV_NOE | isotropic | sample_conditions_1 |
3D 1H-15N NOESY (HCN) | ILV_NOE | isotropic | sample_conditions_1 |
3D 1H-15N NOESY (HNH) | 2D | isotropic | sample_conditions_1 |
3D 1H-15N NOESY (NNH) | 2D | isotropic | sample_conditions_1 |
SIM-HMCM(CGCBCA)CO | ILV_CC | isotropic | sample_conditions_1 |
HMCM(CG)CBCA | ILV_CC | isotropic | sample_conditions_1 |
Software:
nmrPipe, Delaglio, Zhengrong and Bax - data analysis
sparky, Goddard - chemical shift assignment
TOPSPIN v3.1, Bruker Biospin - collection
CS-Rosetta v3.0, Shen, Vernon, Baker and Bax - structure solution
NMR spectrometers:
- Bruker Avance 900 MHz
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts