BMRB Entry 19769
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19769
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Title: Backbone Chemical Shifts of the designed protein Z-L2LBT variant A
Deposition date: 2014-02-05 Original release date: 2015-12-29
Authors: Subedi, Ganesh; Barb, Adam
Citation: Subedi, Ganesh; Barb, Adam. "An encodable lanthanide binding tag with reduced size and flexibility for measuring residual dipolar couplings and pseudocontact shifts in large proteins" J. Biomol. NMR ., .-..
Assembly members:
Z-L2LBT_variant_A, polymer, 83 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Z-L2LBT_variant_A: MGSSHHHHHHSSGVDNKFNK
EQQNAFYEILHLPNLNEEQR
NAFIQSLKDYIDTNNDGAYE
GDELQSADLLAEAKKLNDAQ
APK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 161 |
15N chemical shifts | 61 |
1H chemical shifts | 61 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Z-L2LBT variant A | 1 |
Entities:
Entity 1, Z-L2LBT variant A 83 residues - Formula weight is not available
1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
2 | SER | SER | GLY | VAL | ASP | ASN | LYS | PHE | ASN | LYS | ||||
3 | GLU | GLN | GLN | ASN | ALA | PHE | TYR | GLU | ILE | LEU | ||||
4 | HIS | LEU | PRO | ASN | LEU | ASN | GLU | GLU | GLN | ARG | ||||
5 | ASN | ALA | PHE | ILE | GLN | SER | LEU | LYS | ASP | TYR | ||||
6 | ILE | ASP | THR | ASN | ASN | ASP | GLY | ALA | TYR | GLU | ||||
7 | GLY | ASP | GLU | LEU | GLN | SER | ALA | ASP | LEU | LEU | ||||
8 | ALA | GLU | ALA | LYS | LYS | LEU | ASN | ASP | ALA | GLN | ||||
9 | ALA | PRO | LYS |
Samples:
sample_1: Z-L2LBT variant A, [U-98% 13C; U-98% 15N], 150 uM; 3-(N-morpholino)propanesulfonic acid (MOPS) 25 mM; sodium chloride 100 mM; 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS) 0.5 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 8.0; pressure: 1 atm; temperature: 283.15 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.115, Goddard - chemical shift assignment
NMR spectrometers:
- Varian VXRS 900 MHz
- Varian VXRS 800 MHz
- Bruker Avance 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts