BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19808

Title: Ginsentides: Characterization, Structure and Application of a New Class of Highly Stable Cystine Knot Peptides in Ginseng

Deposition date: 2014-02-20 Original release date: 2015-03-02

Authors: Wang, Shujing; Nguyen, Kien Truc Giang; Luo, Shining; Tam, James P.; Yang, Daiwen

Citation: Wang, Shujing; Nguyen, Kien Truc Giang; Luo, Shining; Tam, James P.; Yang, Daiwen. "Ginsentides: Characterization, Structure and Application of a New Class of Highly Stable Cystine Knot Peptides in Ginseng"  Not known ., .-..

Assembly members:
entity, polymer, 31 residues, 3065.522 Da.

Natural source:   Common Name: Ginseng   Taxonomy ID: 4054   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Panax ginseng

Experimental source:   Production method: purified from the natural source   Host organism: Panax ginseng

Entity Sequences (FASTA):
entity: CKSGGAWCGFDPHGCCGNCG CLVGFCYGTGC

Data sets:
Data typeCount
1H chemical shifts162

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ginsentide1

Entities:

Entity 1, Ginsentide 31 residues - 3065.522 Da.

1   CYSLYSSERGLYGLYALATRPCYSGLYPHE
2   ASPPROHISGLYCYSCYSGLYASNCYSGLY
3   CYSLEUVALGLYPHECYSTYRGLYTHRGLY
4   CYS

Samples:

sample_1: Ginsentide mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0 M; pH: 3.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

NMRspy, Zheng Yu, Xu Yingqi and Yang Daiwen - chemical shift assignment, peak picking

ProcheckNMR, Laskowski and MacArthur - refinement

Molmol, Koradi, Billeter and Wuthrich - geometry optimization

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAX40471