BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19844

Title: 1H, 13C, and 15N backbone assignment of human ribonuclease 4

Deposition date: 2014-03-07 Original release date: 2014-04-07

Authors: Gagne, Donald; Doucet, Nicolas

Citation: Gagne, Donald; Doucet, Nicolas. "1H, 13C, and 15N backbone assignment of human ribonuclease 4"  J. Biomol. NMR ., .-..

Assembly members:
RNase_4, polymer, 119 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RNase_4: QDGMYQRFLRQHVHPEETGG SDRYCNLMMQRRKMTLYHCK RFNTFIHEDIWNIRSICSTT NIQCKNGKMNCHEGVVKVTD CRDTGSSRAPNCRYRAIAST RRVVIACEGNPQVPVHFDG

Data sets:
Data typeCount
13C chemical shifts210
15N chemical shifts110
1H chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNase 41

Entities:

Entity 1, RNase 4 119 residues - Formula weight is not available

1   GLNASPGLYMETTYRGLNARGPHELEUARG
2   GLNHISVALHISPROGLUGLUTHRGLYGLY
3   SERASPARGTYRCYSASNLEUMETMETGLN
4   ARGARGLYSMETTHRLEUTYRHISCYSLYS
5   ARGPHEASNTHRPHEILEHISGLUASPILE
6   TRPASNILEARGSERILECYSSERTHRTHR
7   ASNILEGLNCYSLYSASNGLYLYSMETASN
8   CYSHISGLUGLYVALVALLYSVALTHRASP
9   CYSARGASPTHRGLYSERSERARGALAPRO
10   ASNCYSARGTYRARGALAILEALASERTHR
11   ARGARGVALVALILEALACYSGLUGLYASN
12   PROGLNVALPROVALHISPHEASPGLY

Samples:

sample_1: RNase 4, [U-13C; U-15N], 0.56 mM; D2O 100%

sample_conditions_1: pH: 5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Agilent OMEGA 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts