BMRB Entry 19922
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19922
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments of human TDP-43 RRM2
Deposition date: 2014-04-18 Original release date: 2014-05-02
Authors: Rabdano, Sevastyan; Podkorytov, Ivan; Yuwen, Tairan; Groves, Adam; Chamberlain, Kyle; Yakimov, Alexander; Skrynnikov, Nikolai
Citation: Rabdano, Sevastyan; Podkorytov, Ivan; Yuwen, Tairan; Groves, Adam; Chamberlain, Kyle; Yakimov, Alexander; Skrynnikov, Nikolai. "Backbone 1H, 13C, and 15N Chemical Shift Assignments of human TDP-43 RRM2" Not known ., .-..
Assembly members:
TDP-43_RRM2, polymer, 76 residues, 8703 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
TDP-43_RRM2: MRKVFVGRCTEDMTEDELRE
FFSQYGDVMDVFIPKPFRAF
AFVTFADDQIAQSLCGEDLI
IKGISVHISNAEPKHN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 221 |
| 1H chemical shifts | 69 |
| 15N chemical shifts | 69 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TDP-43 RRM2, 1 | 1 |
| 2 | TDP-43 RRM2, 2 | 1 |
Entities:
Entity 1, TDP-43 RRM2, 1 76 residues - 8703 Da.
| 1 | MET | ARG | LYS | VAL | PHE | VAL | GLY | ARG | CYS | THR | ||||
| 2 | GLU | ASP | MET | THR | GLU | ASP | GLU | LEU | ARG | GLU | ||||
| 3 | PHE | PHE | SER | GLN | TYR | GLY | ASP | VAL | MET | ASP | ||||
| 4 | VAL | PHE | ILE | PRO | LYS | PRO | PHE | ARG | ALA | PHE | ||||
| 5 | ALA | PHE | VAL | THR | PHE | ALA | ASP | ASP | GLN | ILE | ||||
| 6 | ALA | GLN | SER | LEU | CYS | GLY | GLU | ASP | LEU | ILE | ||||
| 7 | ILE | LYS | GLY | ILE | SER | VAL | HIS | ILE | SER | ASN | ||||
| 8 | ALA | GLU | PRO | LYS | HIS | ASN |
Samples:
sample_1: TDP-43 RRM2, [U-13C; U-15N], 1.5 mM; H2O 90%; D2O, [U-2H], 10%; sodium phosphate 20 mM; DTT 22 mM; sodium azide 0.2%
sample_conditions_1: temperature: 298 K; pH: 6.7; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.2, Bruker Biospin - collection, processing
CARA v1.9.0.0, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance-III 500 MHz
Related Database Links:
| BMRB | 19290 |
| PDB | |
| DBJ | BAD96474 BAE87159 BAG35326 BAG58707 BAG61425 |
| EMBL | CAB43367 CAG38565 CAH92854 CAL37794 |
| GB | AAA70033 AAH71657 AAH95435 ABO32290 ABO32291 |
| REF | NP_001127597 NP_031401 XP_001102660 XP_002750326 XP_003274288 |
| SP | Q13148 Q5R5W2 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts