BMRB Entry 19991
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19991
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Title: NMR assignement of salt stabilised Yeast Frataxin YFH1. PubMed: 25988154
Deposition date: 2014-05-28 Original release date: 2015-10-22
Authors: Vilanova, Bartolome; Sanfelice, Domenico; Martorell, Gabriel; Pastore, Annalisa; Temussi, Piero
Citation: Vilanova, Bartolome; Sanfelice, Domenico; Martorell, Gabriel; Temussi, Piero; Pastore, Annalisa. "Trapping a salt-dependent unfolding intermediate of the marginally stable protein Yfh1" Front. Mol. Biosci. ., .-. (2014).
Assembly members:
Yeast_Frataxin_Yfh1, polymer, 123 residues, 13783.4 Da.
Natural source: Common Name: E. Coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Yeast_Frataxin_Yfh1: MESSTDGQVVPQEVLNLPLE
KYHEEADDYLDHLLDSLEEL
SEAHPDCIPDVELSHGVMTL
EIPAFGTYVINKQPPNKQIW
LASPLSGPNRFDLLNGEWVS
LRNGTKLTDILTEEVEKAIS
KSQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 486 |
| 15N chemical shifts | 110 |
| 1H chemical shifts | 752 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | yeast frataxin | 1 |
Entities:
Entity 1, yeast frataxin 123 residues - 13783.4 Da.
| 1 | MET | GLU | SER | SER | THR | ASP | GLY | GLN | VAL | VAL | ||||
| 2 | PRO | GLN | GLU | VAL | LEU | ASN | LEU | PRO | LEU | GLU | ||||
| 3 | LYS | TYR | HIS | GLU | GLU | ALA | ASP | ASP | TYR | LEU | ||||
| 4 | ASP | HIS | LEU | LEU | ASP | SER | LEU | GLU | GLU | LEU | ||||
| 5 | SER | GLU | ALA | HIS | PRO | ASP | CYS | ILE | PRO | ASP | ||||
| 6 | VAL | GLU | LEU | SER | HIS | GLY | VAL | MET | THR | LEU | ||||
| 7 | GLU | ILE | PRO | ALA | PHE | GLY | THR | TYR | VAL | ILE | ||||
| 8 | ASN | LYS | GLN | PRO | PRO | ASN | LYS | GLN | ILE | TRP | ||||
| 9 | LEU | ALA | SER | PRO | LEU | SER | GLY | PRO | ASN | ARG | ||||
| 10 | PHE | ASP | LEU | LEU | ASN | GLY | GLU | TRP | VAL | SER | ||||
| 11 | LEU | ARG | ASN | GLY | THR | LYS | LEU | THR | ASP | ILE | ||||
| 12 | LEU | THR | GLU | GLU | VAL | GLU | LYS | ALA | ILE | SER | ||||
| 13 | LYS | SER | GLN |
Samples:
sample_1: Yeast Frataxin, Yfh1, [U-98% 13C; U-98% 15N], 1 ± 0.1 mM; HEPES 20 mM; NaCl 100 mM; TCEP 2 mM; Yfh1 1 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 298.2 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 700 MHz
- Bruker Avance 800 MHz
Related Database Links:
| BMRB | 17068 17641 |
| PDB | |
| DBJ | GAA22128 |
| EMBL | CAA98688 CAY78388 |
| GB | AAS56486 AHY74893 AJP37633 AJU57746 AJU58449 |
| REF | NP_010163 |
| SP | Q07540 |
| TPG | DAA11740 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts