BMRB Entry 20002
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20002
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Title: Solution structure of RgIA PubMed: 18242183
Deposition date: 2007-12-20 Original release date: 2008-06-30
Authors: Daly, Norelle; Craik, David
Citation: Clark, Richard; Daly, Norelle; Halai, Reena; Nevin, Simon; Adams, David; Craik, David. "The three-dimensional structure of the analgesic alpha-conotoxin, RgIA" FEBS Lett. 582, 597-602 (2008).
Assembly members:
RgIA, polymer, 13 residues, Formula weight is not available
Natural source: Common Name: Conus regius Taxonomy ID: 101314 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Conus regius
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
RgIA: GCCSDPRCRYRCR
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 77 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | RgIA | 1 |
Entities:
Entity 1, RgIA 13 residues - Formula weight is not available
1 | GLY | CYS | CYS | SER | ASP | PRO | ARG | CYS | ARG | TYR | ||||
2 | ARG | CYS | ARG |
Samples:
sample_1: RgIA 1 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 3.5; temperature: 280 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
SPARKY, Goddard - data analysis
CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 600 MHz