BMRB Entry 20026
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20026
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Title: Mutagenesis and nuclear magnetic resonance analyses of the fusion peptide of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus F protein PubMed: 18524820
Deposition date: 2008-06-26 Original release date: 2008-09-10
Authors: Tan, Y.; Jiang, L.; Wang, M.L.; Yin, F.F.; Deng, F.; Liu, M.L.; Hu, Z.H.; Wang, H.L.
Citation: Tan, Y.; Jiang, L.; Wang, M.L.; Yin, F.F.; Deng, F.; Liu, M.L.; Hu, Z.H.; Wang, H.L.. "Mutagenesis and nuclear magnetic resonance analyses of the fusion peptide of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus F protein" J. Virol. 82, 8138-8148 (2008).
Assembly members:
F protein, polymer, 20 residues, 2144.487 Da.
Natural source: Common Name: cotton bollworm Taxonomy ID: 29058 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Helicoverpa armigera
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
F protein: NIGLNFVGSVDKFLFGVMDA
- assigned_chemical_shifts
- conformer_family_coord_set
- representative_conformer
Data type | Count |
1H chemical shifts | 137 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | F protein | 1 |
Entities:
Entity 1, F protein 20 residues - 2144.487 Da.
1 | ASN | ILE | GLY | LEU | ASN | PHE | VAL | GLY | SER | VAL | |
2 | ASP | LYS | PHE | LEU | PHE | GLY | VAL | MET | ASP | ALA |
Samples:
sample_1: entity 2 mM; sodium phosphate 20 mM; SDS, [U-100% 2H], 200 mM
sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 300 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - chemical shift assignment
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Varian INOVA 600 MHz