BMRB Entry 20029
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20029
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Title: 13C and 1H atom assignments and structure for Apelin 17 (Human form) at 35C PubMed: 19123778
Deposition date: 2008-07-02 Original release date: 2009-02-04
Authors: Langelaan, David; Bebbington, E.; Reddy, Tyler; Rainey, Jan
Citation: Langelaan, David; Bebbington, E.; Reddy, Tyler; Rainey, Jan. "Structural insight into G-protein coupled receptor binding by apelin" Biochemistry 48, 537-548 (2009).
Assembly members:
Apelin_17, polymer, 17 residues, 2145.619 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Apelin_17: KFRRQRPRLSHKGPMPF
- assigned_chemical_shifts
- coupling_constants
Data type | Count |
1H chemical shifts | 131 |
13C chemical shifts | 70 |
coupling constants | 11 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Apelin 17 | 1 |
Entities:
Entity 1, Apelin 17 17 residues - 2145.619 Da.
1 | LYS | PHE | ARG | ARG | GLN | ARG | PRO | ARG | LEU | SER | ||||
2 | HIS | LYS | GLY | PRO | MET | PRO | PHE |
Samples:
Apelin_17_sample: Apelin 17 4 ± 0.4 mM; DSS 1 mM; sodium azide 1 mM; Sodium Acetate, [U-99% 2H], 20 mM
35C: pH: 5; temperature: 308 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-13C HSQC | Apelin_17_sample | isotropic | 35C |
2D 1H-1H TOCSY | Apelin_17_sample | isotropic | 35C |
2D 1H-1H NOESY | Apelin_17_sample | isotropic | 35C |
2D DQF-COSY | Apelin_17_sample | isotropic | 35C |
Software:
VNMR, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment, peak picking
TOPSPIN, Bruker Biospin - collection
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
- Bruker Avance 500 MHz