BMRB Entry 20102
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20102
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Title: C-Terminal helix of the SERCA2b Ca2+ pump (ATP2A2B) PubMed: 19846779
Deposition date: 2009-09-11 Original release date: 2009-10-09
Authors: Vandecaetsbeek, Ilse; Trekels, Mieke; De Maeyer, Marc; Ceulemans, Hugo; Lescrinier, Eveline; Raeymaekers, Luc; Wuytack, Frank; Vangheluwe, Peter
Citation: Vandecaetsbeek, Ilse; Trekels, Mieke; De Maeyer, Marc; Ceulemans, Hugo; Lescrinier, Eveline; Raeymaekers, Luc; Wuytack, Frank; Vangheluwe, Peter. "Structural basis for the high Ca2+ affinity of the ubiquitous SERCA2b Ca2+ pump." Proc. Natl. Acad. Sci. U.S.A. 106, 18533-18538 (2009).
Assembly members:
t11, polymer, 20 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
t11: DGISWPFVLLIMPLVIWVYS
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 133 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | t11 | 1 |
Entities:
Entity 1, t11 20 residues - Formula weight is not available
1 | ASP | GLY | ILE | SER | TRP | PRO | PHE | VAL | LEU | LEU | |
2 | ILE | MET | PRO | LEU | VAL | ILE | TRP | VAL | TYR | SER |
Samples:
sample_1: t11 3 mM; CDCL3 50%; DMSO 50%
sample_conditions_1: pH: 7; temperature: 295 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, torsion angle dynamics
TOPSPIN, Bruker Biospin - collection
CARA, Rochus Keller, Kurt W thrich - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 600 MHz