BMRB Entry 20109
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20109
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Title: CHARACTERIZATION OF THE LIGAND-FREE CONSTITUTIVE ACTIVITY OF THE THROMBOXANE A2 RECEPTOR BY THE INTEGRATED APPROACHES OF NMR SPECTROSCOPY AND A RECOMBINANT RECEPTOR PubMed: 20590159
Deposition date: 2009-12-09 Original release date: 2011-02-14
Authors: Chillar, Annirudha; Wu, Jiaxin; Cervantes, Vanessa; Ruan, Ke-He
Citation: Chillar, Annirudha; Wu, Jiaxin; Cervantes, Vanessa; Ruan, Ke-He. "Structural and functional analysis of the C-terminus of Galphaq in complex with the human thromboxane A2 receptor provides evidence of constitutive activity" Biochemistry 49, 6365-6374 (2010).
Assembly members:
Gaq-Ct peptide, polymer, 15 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Gaq-Ct peptide: KDTILQLNLKEYNLV
- assigned_chemical_shifts
- conformer_family_coord_set
Data type | Count |
1H chemical shifts | 104 |
conformer coordinate sets | 1 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Gaq-Ct peptide | 1 |
Entities:
Entity 1, Gaq-Ct peptide 15 residues - Formula weight is not available
1 | LYS | ASP | THR | ILE | LEU | GLN | LEU | ASN | LEU | LYS | ||||
2 | GLU | TYR | ASN | LEU | VAL |
Samples:
sample_1: Gaq-Ct peptide 1.1 mM; H2O 95%; D2O 5%
sample_conditions_1: temperature: 293 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
FELIX v2000, Accelrys Software Inc. - chemical shift assignment, chemical shift calculation, data analysis, peak picking, processing, refinement, structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
BMRB | 20112 20118 |
EMBL | CAG08422 |
GB | ACF47673 ACF47675 EGW10015 EGW13773 EHB02791 |
REF | XP_004048208 XP_004059801 XP_005431300 XP_005642455 XP_008508489 |