BMRB Entry 20111
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20111
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Title: Conformational Flexibility of SecA IRA1 Loop Region PubMed: 21337475
Deposition date: 2009-12-10 Original release date: 2011-06-02
Authors: Palladino, Pasquale; Saviano, Gabriella; Tancredi, Teodorico; Ragone, Raffaele; Benedetti, Ettore; Rossi, Filomena
Citation: Palladino, Pasquale; Saviano, Gabriella; Tancredi, Teodorico; Benedetti, Ettore; Rossi, Filomena; Ragone, Raffaele. "Structural determinants of protein translocation in bacteria: conformational flexibility of SecA IRA1 loop region." J. Pept. Sci. 17, 263-269 (2011).
Assembly members:
SecA[788-804]Y794A, polymer, 17 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SecA[788-804]Y794A: GIHLRGAAQKDPKQEYK
- assigned_chemical_shifts
- conformer_family_coord_set
- spectral_peak_list
Data type | Count |
1H chemical shifts | 120 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | SecA fragment | 1 |
Entities:
Entity 1, SecA fragment 17 residues - Formula weight is not available
1 | GLY | ILE | HIS | LEU | ARG | GLY | ALA | ALA | GLN | LYS | ||||
2 | ASP | PRO | LYS | GLN | GLU | TYR | LYS |
Samples:
sample_1: SecA[788-804]Y794A 1 mM; TFEd2-OH, [U-100% 2H], 50 % v/v; H2O 50 % v/v
sample_conditions_1: pH: 5.1; temperature: 300 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz