BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 21001

Title: NMR solution of a tridimensional peptidic structure in bilayer lipids: Example with neurotensin in Small Unilamellar Vesicle environment   PubMed: 22928939

Deposition date: 2010-12-09 Original release date: 2012-07-25

Authors: Monti, Jean-Pierre; Da Costa, Gregory; Bondon, Arnaud

Citation: Da Costa, Gregory; Bondon, Arnaud; Delalande, Olivier; Mouret, Liza; Monti, Jean-Pierre. "Elucidation by NMR solution of neurotensin in small unilamellar vesicle environment: molecular surveys for neurotensin receptor recognition"  J. Biomol. Struct. Dyn. ., .-. (2012).

Assembly members:
neurotensin, polymer, 13 residues, 1672.95 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
neurotensin: XLYENKPRRPYIL

Data sets:
Data typeCount
1H chemical shifts109

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1neurotensin1

Entities:

Entity 1, neurotensin 13 residues - 1672.95 Da.

1   PCALEUTYRGLUASNLYSPROARGARGPRO
2   TYRILELEU

Samples:

sample_1: neurotensin 0.6 mM; Small Unilamellar Vesicle 36 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.3; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSY No.1sample_1isotropicsample_conditions_1
2D 1H-1H NOESY No.1sample_1isotropicsample_conditions_1
2D 1H-1H TOCSY No.2sample_1isotropicsample_conditions_1
2D 1H-1H NOESY No.2sample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - processing

SPARKY, Goddard - data analysis

InsightII, Accelrys Software Inc. - structure solution

ProcheckNMR, Laskowski and MacArthur - data analysis

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz