BMRB Entry 25118
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25118
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Title: Chemical shift assignments for human cardiac troponin I, residues 1-73
Deposition date: 2014-07-31 Original release date: 2014-09-03
Authors: Hwang, Peter
Citation: Hwang, Peter. "The intrinsically disordered cardiac-specific N-terminal region of troponin I positions the regulatory domain of troponin C" Proc. Natl. Acad. Sci. U.S.A. ., .-..
Assembly members:
cTnI[1-73], polymer, 80 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
cTnI[1-73]: LADGSSDAAREPRPAPAPIR
RRSSNYRAYATEPHAKKKSK
ISASRKLQLKTLLLQIAKQE
LEREAEERRGEKGRHHHHHH
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 300 |
13C chemical shifts | 227 |
15N chemical shifts | 66 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | cTnI[1-73] | 1 |
Entities:
Entity 1, cTnI[1-73] 80 residues - Formula weight is not available
1 | LEU | ALA | ASP | GLY | SER | SER | ASP | ALA | ALA | ARG | |
2 | GLU | PRO | ARG | PRO | ALA | PRO | ALA | PRO | ILE | ARG | |
3 | ARG | ARG | SER | SER | ASN | TYR | ARG | ALA | TYR | ALA | |
4 | THR | GLU | PRO | HIS | ALA | LYS | LYS | LYS | SER | LYS | |
5 | ILE | SER | ALA | SER | ARG | LYS | LEU | GLN | LEU | LYS | |
6 | THR | LEU | LEU | LEU | GLN | ILE | ALA | LYS | GLN | GLU | |
7 | LEU | GLU | ARG | GLU | ALA | GLU | GLU | ARG | ARG | GLY | |
8 | GLU | LYS | GLY | ARG | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: cTnI[1-73], [U-100% 13C; U-100% 15N], 0.5 mM; H2O 95%; D2O 5%
sample_conditions_1: temperature: 313 K; pH: 6.2; pressure: 1 atm; ionic strength: 0.12 M
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
BMRB | 25119 |
DBJ | BAD97171 |
EMBL | CAA38102 CAA62301 CAG46782 CAG46809 |
GB | AAA16157 AAH96165 AAH96166 AAH96167 AAH99631 |
PRF | 1616225A |
REF | NP_000354 XP_003807165 XP_004061509 XP_009434690 |
SP | P19429 |
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