BMRB Entry 25182
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25182
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Title: Backbone chemical shift assignment of FG-NUP (274 -399) under in-cell condition.
Deposition date: 2014-08-29 Original release date: 2015-10-08
Authors: Dutta, Kaushik; Cowburn, David; Rout, Michael
Citation: Hough, Loren; Dutta, Kaushik; Sparks, Sam; Temel, Deniz; Kamal, Alia; Tetenbaum-Novatt, Jaclyn; Rout, Michael; Cowburn, David. "Atomic Scale Dynamic Behavior of the Nuclear Transport Selectivity Barrier" Science ., .-..
Assembly members:
FSFG-K, polymer, 125 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
FSFG-K: MDNKTTNTTPSFSFGAKSDE
NKAGATSKPAFSFGAKPEEK
KDDNSSKPAFSFGAKSNEDK
QDGTAKPAFSFGAKPAEKNN
NETSKPAFSFGAKSDEKKDG
DASKPAFSFGAKPDENKASA
TSKPA
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 350 |
15N chemical shifts | 109 |
1H chemical shifts | 109 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | entity | 1 |
Entities:
Entity 1, entity 125 residues - Formula weight is not available
1 | MET | ASP | ASN | LYS | THR | THR | ASN | THR | THR | PRO | ||||
2 | SER | PHE | SER | PHE | GLY | ALA | LYS | SER | ASP | GLU | ||||
3 | ASN | LYS | ALA | GLY | ALA | THR | SER | LYS | PRO | ALA | ||||
4 | PHE | SER | PHE | GLY | ALA | LYS | PRO | GLU | GLU | LYS | ||||
5 | LYS | ASP | ASP | ASN | SER | SER | LYS | PRO | ALA | PHE | ||||
6 | SER | PHE | GLY | ALA | LYS | SER | ASN | GLU | ASP | LYS | ||||
7 | GLN | ASP | GLY | THR | ALA | LYS | PRO | ALA | PHE | SER | ||||
8 | PHE | GLY | ALA | LYS | PRO | ALA | GLU | LYS | ASN | ASN | ||||
9 | ASN | GLU | THR | SER | LYS | PRO | ALA | PHE | SER | PHE | ||||
10 | GLY | ALA | LYS | SER | ASP | GLU | LYS | LYS | ASP | GLY | ||||
11 | ASP | ALA | SER | LYS | PRO | ALA | PHE | SER | PHE | GLY | ||||
12 | ALA | LYS | PRO | ASP | GLU | ASN | LYS | ALA | SER | ALA | ||||
13 | THR | SER | LYS | PRO | ALA |
Samples:
sample_1: FSFG-K, [U-100% 15N], 500 uM; H2O 90%; D2O 10%
sample_2: FSFG-K, [U-100% 13C; U-100% 15N], 500 uM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 20 mM; pH: 7.3; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_2 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
3D HNCA | sample_2 | isotropic | sample_conditions_1 |
3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_2 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_2 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 800 MHz
- Bruker Avance 900 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts