BMRB Entry 25197
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25197
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Title: wrapping silk W2 PubMed: 26112753
Deposition date: 2014-09-03 Original release date: 2015-09-04
Authors: Tremblay, Marie-Laurence; Rainey, Jan; Liu, Xiang-Qin; Xu, Lingling; Meng, Qing
Citation: Tremblay, Marie-Laurence; Xu, Lingling; Lefevre, Thierry; Sarker, Muzaddid; Orrell, Kathleen; Leclerc, Jereie; Meng, Qing; Pezolet, Michel; Auger, Michele; Liu, Xiang-Qin; Rainey, Jan. "Spider wrapping silk fibre architecture arising from its modular soluble protein precursor" Sci. Rep. 5, 11502-11502 (2015).
Assembly members:
W2, polymer, 399 residues, 38022.2092 Da.
Natural source: Common Name: spider Taxonomy ID: 156845 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Argiope trifasciata
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 786 |
15N chemical shifts | 374 |
1H chemical shifts | 374 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | AcSp1 W2 | 1 |
Entities:
Entity 1, AcSp1 W2 399 residues - 38022.2092 Da.
as is
1 | ALA | GLY | PRO | GLN | GLY | GLY | PHE | GLY | ALA | THR | ||||
2 | GLY | GLY | ALA | SER | ALA | GLY | LEU | ILE | SER | ARG | ||||
3 | VAL | ALA | ASN | ALA | LEU | ALA | ASN | THR | SER | THR | ||||
4 | LEU | ARG | THR | VAL | LEU | ARG | THR | GLY | VAL | SER | ||||
5 | GLN | GLN | ILE | ALA | SER | SER | VAL | VAL | GLN | ARG | ||||
6 | ALA | ALA | GLN | SER | LEU | ALA | SER | THR | LEU | GLY | ||||
7 | VAL | ASP | GLY | ASN | ASN | LEU | ALA | ARG | PHE | ALA | ||||
8 | VAL | GLN | ALA | VAL | SER | ARG | LEU | PRO | ALA | GLY | ||||
9 | SER | ASP | THR | SER | ALA | TYR | ALA | GLN | ALA | PHE | ||||
10 | SER | SER | ALA | LEU | PHE | ASN | ALA | GLY | VAL | LEU | ||||
11 | ASN | ALA | SER | ASN | ILE | ASP | THR | LEU | GLY | SER | ||||
12 | ARG | VAL | LEU | SER | ALA | LEU | LEU | ASN | GLY | VAL | ||||
13 | SER | SER | ALA | ALA | GLN | GLY | LEU | GLY | ILE | ASN | ||||
14 | VAL | ASP | SER | GLY | SER | VAL | GLN | SER | ASP | ILE | ||||
15 | SER | SER | SER | SER | SER | PHE | LEU | SER | THR | SER | ||||
16 | SER | SER | SER | ALA | SER | TYR | SER | GLN | ALA | SER | ||||
17 | ALA | SER | SER | THR | SER | GLY | ALA | GLY | TYR | THR | ||||
18 | GLY | PRO | SER | GLY | PRO | SER | THR | GLY | PRO | SER | ||||
19 | GLY | TYR | PRO | GLY | PRO | LEU | GLY | GLY | GLY | ALA | ||||
20 | PRO | PHE | GLY | GLN | SER | GLY | PHE | GLY | GLY | SER | ||||
21 | ALA | GLY | PRO | GLN | GLY | GLY | PHE | GLY | ALA | THR | ||||
22 | GLY | GLY | ALA | SER | ALA | GLY | LEU | ILE | SER | ARG | ||||
23 | VAL | ALA | ASN | ALA | LEU | ALA | ASN | THR | SER | THR | ||||
24 | LEU | ARG | THR | VAL | LEU | ARG | THR | GLY | VAL | SER | ||||
25 | GLN | GLN | ILE | ALA | SER | SER | VAL | VAL | GLN | ARG | ||||
26 | ALA | ALA | GLN | SER | LEU | ALA | SER | THR | LEU | GLY | ||||
27 | VAL | ASP | GLY | ASN | ASN | LEU | ALA | ARG | PHE | ALA | ||||
28 | VAL | GLN | ALA | VAL | SER | ARG | LEU | PRO | ALA | GLY | ||||
29 | SER | ASP | THR | SER | ALA | TYR | ALA | GLN | ALA | PHE | ||||
30 | SER | SER | ALA | LEU | PHE | ASN | ALA | GLY | VAL | LEU | ||||
31 | ASN | ALA | SER | ASN | ILE | ASP | THR | LEU | GLY | SER | ||||
32 | ARG | VAL | LEU | SER | ALA | LEU | LEU | ASN | GLY | VAL | ||||
33 | SER | SER | ALA | ALA | GLN | GLY | LEU | GLY | ILE | ASN | ||||
34 | VAL | ASP | SER | GLY | SER | VAL | GLN | SER | ASP | ILE | ||||
35 | SER | SER | SER | SER | SER | PHE | LEU | SER | THR | SER | ||||
36 | SER | SER | SER | ALA | SER | TYR | SER | GLN | ALA | SER | ||||
37 | ALA | SER | SER | THR | SER | GLY | ALA | GLY | TYR | THR | ||||
38 | GLY | PRO | SER | GLY | PRO | SER | THR | GLY | PRO | SER | ||||
39 | GLY | TYR | PRO | GLY | PRO | LEU | GLY | GLY | GLY | ALA | ||||
40 | PRO | PHE | GLY | GLN | SER | GLY | PHE | GLY | GLY |
Samples:
W2-1: W2, [U-13C; U-15N], 0.2 mM; DSS 1 mM; sodium azide 1 mM; sodium acetate, [U-2H], 20 mM
W2-2: W2, [U-13C; U-15N], 0.2 mM; DSS 1 mM; sodium azide 1 mM; sodium acetate, [U-2H], 20 mM
sample1: ionic strength: 0.100 M; pH: 5.020; pressure: 1.000 atm; temperature: 303.150 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCO | W2-1 | isotropic | sample1 |
3D HN(CO)CA | W2-1 | isotropic | sample1 |
3D HNCA | W2-1 | isotropic | sample1 |
2D 1H-15N HSQC/HMQC | W2-1 | isotropic | sample1 |
3D HN(CA)CO | W2-1 | isotropic | sample1 |
2D 1H-15N HSQC/HMQC | W2-2 | isotropic | sample1 |
3D HNCA | W2-2 | isotropic | sample1 |
3D HN(CO)CA | W2-2 | isotropic | sample1 |
3D HN(CA)CO | W2-2 | isotropic | sample1 |
3D HNCO | W2-2 | isotropic | sample1 |
Software:
CcpNmr_Analysis v2.2, CCPN - Spectrum display, data Analysis
NMRpipe v1.0, Dr. Delaglio and Dr. Bax - Data processing
NMR spectrometers:
- Bruker Avance 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts