BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25293

Title: Kalata B7 Ser mutant

Deposition date: 2014-10-22 Original release date: 2015-12-21

Authors: Kristiansen, Per Eugen; Skjeldal, Lars; Goransson, Ulf

Citation: Kristiansen, Per Eugen; Skjeldal, Lars; Goransson, Ulf. "Kalata B7 Ser mutant"  To be Published ., .-..

Assembly members:
entity, polymer, 29 residues, 3021.515 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis   Host organism: not applicable

Entity Sequences (FASTA):
entity: SWPICKRNGLPVCGETCTLG TCSTQGCTC

Data sets:
Data typeCount
13C chemical shifts70
15N chemical shifts22
1H chemical shifts178

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 29 residues - 3021.515 Da.

1   SERTRPPROILECYSLYSARGASNGLYLEU
2   PROVALCYSGLYGLUTHRCYSTHRLEUGLY
3   THRCYSSERTHRGLNGLYCYSTHRCYS

Samples:

sample_1: Kalata B7 mutant 1 mM; H2O 5%; D2O, [U-100% 2H], 95%; DSS 0.2 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

TOPSPIN v2.4, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts