BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25314

Title: FBP28 WW2 mutant Y238R L453A DNDC   PubMed: 25489078

Deposition date: 2014-11-04 Original release date: 2014-11-24

Authors: Macias, Maria; Scheraga, Harold; Sunol, David; Todorovski, Toni

Citation: Zhou, Rui; Maisuradze, Gia; Sunol, David; Todorovski, Toni; Macias, Maria; Xiao, Yi; Scheraga, Harold; Czaplewski, Cezary; Liwo, Adam. "Folding kinetics of WW domains with the united residue force field for bridging microscopic motions and experimental measurements"  Proc. Natl. Acad. Sci. U.S.A. 111, 18243-18248 (2014).

Assembly members:
entity, polymer, 28 residues, 3417.664 Da.

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: SEWTERKTADGKTYYYNNRT AESTWEKP

Data sets:
Data typeCount
1H chemical shifts168

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 28 residues - 3417.664 Da.

1   SERGLUTRPTHRGLUARGLYSTHRALAASP
2   GLYLYSTHRTYRTYRTYRASNASNARGTHR
3   ALAGLUSERTHRTRPGLULYSPRO

Samples:

sample_1: entity 0.7 mM; sodium chloride 100 mM; sodium phosphate 20 mM; sodium azide 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

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