BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25329

Title: Backbone Assignment Of the homodimer HUB2 from E.coli at 293K   PubMed: 25924603

Deposition date: 2014-11-12 Original release date: 2015-05-19

Authors: Le Meur, Remy; Castaing, Bertrand; Landon, Celine; Loth, Karine

Citation: Le Meur, Remy; Castaing, Bertrand; Landon, Celine; Loth, Karine. "Backbone assignment of the three dimers of HU from Escherichia coli at 293 K: EcHUalpha2, EcHUbeta2 2 and EcHUalphabeta2"  Biomol. NMR Assignments 9, 359-363 (2015).

Assembly members:
HUB2, polymer, 90 residues, Formula weight is not available

Natural source:   Common Name: E.coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HUB2: MNKSQLIDKIAAGADISKAA AGRALDAIIASVTESLKEGD DVALVGFGTFAVKERAARTG RNPQTGKEITIAAAKVPSFR AGKALKDAVN

Data sets:
Data typeCount
13C chemical shifts252
15N chemical shifts85
1H chemical shifts85

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HUB2, chain 11
2HUB2, chain 21

Entities:

Entity 1, HUB2, chain 1 90 residues - Formula weight is not available

1   METASNLYSSERGLNLEUILEASPLYSILE
2   ALAALAGLYALAASPILESERLYSALAALA
3   ALAGLYARGALALEUASPALAILEILEALA
4   SERVALTHRGLUSERLEULYSGLUGLYASP
5   ASPVALALALEUVALGLYPHEGLYTHRPHE
6   ALAVALLYSGLUARGALAALAARGTHRGLY
7   ARGASNPROGLNTHRGLYLYSGLUILETHR
8   ILEALAALAALALYSVALPROSERPHEARG
9   ALAGLYLYSALALEULYSASPALAVALASN

Samples:

sample_1: HUB2, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 20 mM; sodium chloride 200 mM; DTT 0.05 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.22 M; pH: 7.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts