BMRB Entry 25364

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments drosophila stem-loop binding protein complexed with histone mRNA stem-loop
Published: 2014-12-09

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25364

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments drosophila stem-loop binding protein complexed with histone mRNA stem-loop PubMed: 25002523

Deposition date: 2014-11-24 Original release date: 2014-12-09

Authors: Zhang, Jun; Tan, Dazhi; DeRose, Eugene; Perera, Lalith; Dominski, Zbigniew; Marzluff, William; Tong, Liang; Hall, Traci

Citation: Zhang, Jun; Derose, Eugene; Perera, Lalith; Dominski, Zbigniew; Marzluff, William; Tong, Liang; Hall, Traci. "Molecular mechanisms for the regulation of histone mRNA stem-loop binding protein by phosphorylation" Proc. Natl. Acad. Sci. U.S.A. 111, E2937-E2946 (2014).

Assembly members:
SLBP, polymer, 93 residues, Formula weight is not available
RNA, polymer, 28 residues, Formula weight is not available

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
SLBP: SSSYTEADPAILSRRQKQID YGKNTAAYERYVEMVPKDER TRDHPREPNKYGKYSRRAFD GLVKIWRKSLHIYDPPTQAR DTAKDENEDEDED
RNA: GGCCAAAGGCCCUUUUCAGG GCCACCCA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	148
1H chemical shifts	62
15N chemical shifts	62

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SLBP	1
2	RNA	2

Entities:

Entity 1, SLBP 93 residues - Formula weight is not available

1

SER

TYR

THR

GLU

ALA

ASP

PRO

ALA

2

ILE

LEU

SER

ARG

GLN

LYS

GLN

ILE

ASP

3

TYR

GLY

LYS

ASN

THR

ALA

TYR

GLU

ARG

4

TYR

VAL

GLU

MET

VAL

PRO

LYS

ASP

GLU

ARG

5

THR

ARG

ASP

HIS

PRO

ARG

GLU

PRO

ASN

LYS

6

TYR

GLY

LYS

TYR

SER

ARG

ALA

PHE

ASP

7

GLY

LEU

VAL

LYS

ILE

TRP

ARG

LYS

SER

LEU

8

HIS

ILE

TYR

ASP

PRO

THR

GLN

ALA

ARG

9

ASP

THR

ALA

LYS

ASP

GLU

ASN

GLU

ASP

GLU

10

ASP

GLU

ASP

Entity 2, RNA 28 residues - Formula weight is not available

1

G

C

A

G

C

2

C

U

C

A

G

3

G

C

A

C

A

Samples:

sample_1: SLBP, [U-100% 13C; U-100% 15N], 0.8 mM; Hepes 20 mM; NaCl 300 mM

sample_conditions_1: temperature: 273 K; pH: 7.5; pressure: 1 atm; ionic strength: 300 mM

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	25365
PDB	4TUW 4TUX
GB	AAF56867 AAF71752 AAG16723 AAL90413 AAM50696
REF	NP_477480
SP	Q9VAN6

Biological Magnetic Resonance Data Bank