BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25382

Title: UBX-L domain of VCIP135

Deposition date: 2014-12-07 Original release date: 2016-07-11

Authors: Iwazu, Takahiro; Murayama, Shuhei; Igarashi, Ryuji; Hrioaki, Hidekazu; Shirakawa, Masahiro; Tochio, Hidehito

Citation: Iwazu, Takahiro; Murayama, Shuhei; Igarashi, Ryuji; Hrioaki, Hidekazu; Shirakawa, Masahiro; Tochio, Hidehito. "Structure and interaction mode of the UBX-L domain of VCIP135 determined by solution NMR spectroscopy"  Sci. Rep. ., .-..

Assembly members:
VCIP135 UBX, polymer, 86 residues, 9331.919 Da.

Natural source:   Common Name: Norway Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
VCIP135 UBX: GSDHSKEKKIRITTNDGRQS MVTLKSSTTFFELQESIARE FNIPPYLQCIRYGFPPKELM PPQAGMEKEPVPLQHGDRIT IEILKG

Data sets:
Data typeCount
13C chemical shifts316
15N chemical shifts79
1H chemical shifts638

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1VCIP135 UBX1

Entities:

Entity 1, VCIP135 UBX 86 residues - 9331.919 Da.

1   GLYSERASPHISSERLYSGLULYSLYSILE
2   ARGILETHRTHRASNASPGLYARGGLNSER
3   METVALTHRLEULYSSERSERTHRTHRPHE
4   PHEGLULEUGLNGLUSERILEALAARGGLU
5   PHEASNILEPROPROTYRLEUGLNCYSILE
6   ARGTYRGLYPHEPROPROLYSGLULEUMET
7   PROPROGLNALAGLYMETGLULYSGLUPRO
8   VALPROLEUGLNHISGLYASPARGILETHR
9   ILEGLUILELEULYSGLY

Samples:

sample_1: VCIP135 UBX, [U-13C; U-15N], 1.86 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker DRX 700 MHz
  • Bruker DPX 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts